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Title: Production of single-round infectious chimeric flaviviruses with DNA-based Japanese encephalitis virus replicon
Authors: Ryosuke Suzuki
Tomohiro Ishikawa
Eiji Konishi
Mami Matsuda
Koichi Watashi
Hideki Aizaki
Tomohiko Takasaki
Takaji Wakita
National Institute of Infectious Diseases
Dokkyo Medical University
Mahidol University
Keywords: Immunology and Microbiology
Issue Date: 1-Jan-2014
Citation: Journal of General Virology. Vol.95, No.PART 1 (2014), 60-65
Abstract: A method for rapid production of single-round infectious particles (SRIPs) of flavivirus would be useful for viral mutagenesis studies. Here, we established a DNA-based production system for SRIPs of flavivirus. We constructed a Japanese encephalitis virus (JEV) subgenomic replicon plasmid, which lacked the C-prM-E (capsid-pre-membrane-envelope) coding region, under the control of the cytomegalovirus promoter. When the JEV replicon plasmid was transiently co-transfected with a JEV C-prM-E expression plasmid into 293T cells, SRIPs were produced, indicating successful trans-complementation with JEV structural proteins. Equivalent production levels were observed when C and prM-E proteins were provided separately. Furthermore, dengue types 1-4, West Nile, yellow fever or tick-borne encephalitis virus prM-E proteins could be utilized for production of chimaeric flavivirus SRIPs, although the production was less efficient for dengue and yellow fever viruses. These results indicated that our plasmid-based system is suitable for investigating the life cycles of flaviviruses, diagnostic applications and development of safer vaccine candidates. © 2014 SGM.
ISSN: 14652099
Appears in Collections:Scopus 2011-2015

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