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Title: Involvement of ATP synthase β subunit in chikungunya virus entry into insect cells
Authors: Chanida Fongsaran
Krit Jirakanwisal
Atichat Kuadkitkan
Nitwara Wikan
Phitchayapak Wintachai
Chutima Thepparit
Sukathida Ubol
Narumon Phaonakrop
Sittiruk Roytrakul
Duncan R. Smith
Mahidol University
Thailand National Center for Genetic Engineering and Biotechnology
Keywords: Immunology and Microbiology
Issue Date: 1-Jan-2014
Citation: Archives of Virology. Vol.159, No.12 (2014), 3353-3364
Abstract: © 2014, Springer-Verlag Wien. Chikungunya virus (CHIKV), the virus responsible for the disease chikungunya fever in humans, is transmitted by Aedes mosquitoes. While significant progress has been made in understanding the process by which CHIKV enters into mammalian cells, far less progress has been made in understanding the CHIKV entry process in insect cells. This study sought to identify mosquito-cell-expressed CHIKV-binding proteins through a combination of virus overlay protein binding assays (VOPBA) and mass spectroscopy. A 50-kDa CHIKV-binding protein was identified as the ATP synthase β subunit (ATPSβ). Co-immunoprecipitation studies confirmed the interaction, and colocalization analysis showed cell-surface and intracellular co-localization between CHIKV and ATPSβ. Both antibody inhibition and siRNA-mediated downregulation experiments targeted to ATPSβ showed a significant reduction in viral entry and virus production. These results suggest that ATPSβ is a CHIKV-binding protein capable of mediating the entry of CHIKV into insect cells.
ISSN: 03048608
Appears in Collections:Scopus 2011-2015

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