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Title: Nine Human sparganosis cases in Thailand with molecular identification of causative parasite species
Authors: Adhiratha Boonyasiri
Pornsuk Cheunsuchon
Yupin Suputtamongkol
Hiroshi Yamasaki
Oranuch Sanpool
Wanchai Maleewong
Pewpan M. Intapan
Mahidol University
National Institute of Infectious Diseases
Khon Kaen University
Keywords: Immunology and Microbiology;Medicine
Issue Date: 1-Jan-2014
Citation: American Journal of Tropical Medicine and Hygiene. Vol.91, No.2 (2014), 389-393
Abstract: Human sparganosis is one of the neglected diseases but important food-borne parasitic zoonoses. The disease is caused by larvae (spargana) of diphyllobothriidean tapeworm. Here, we describe nine cases of human sparganosis, caused by Spirometra erinaceieuropaei in a hospital in Thailand during 2001-2012. Clinical characteristics, treatment, and outcome of cases were revealed. Diagnosis and identification of causative parasite species was made by histopathological investigations followed by a polymerase chain reaction-based molecular method using formalin-fixed paraffin embedded tissues. The DNA samples were extracted from tissues and a partial fragment of cytochrome c oxidase subunit 1 (cox1) gene was amplified for the detection of parasitic DNA. Infection could be prevented by increasing activities on health communication by responsible public health agencies. Copyright © 2014 by The American Society of Tropical Medicine and Hygiene.
ISSN: 00029637
Appears in Collections:Scopus 2011-2015

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