Please use this identifier to cite or link to this item: http://repository.li.mahidol.ac.th/dspace/handle/123456789/34076
Title: Application of loop-mediated isothermal amplification assay combined with lateral flow dipstick for detection of Plasmodium falciparum and Plasmodium vivax
Authors: Suganya Yongkiettrakul
Wansadaj Jaroenram
Narong Arunrut
Wanwisa Chareanchim
Supicha Pannengpetch
Rungkarn Suebsing
Wansika Kiatpathomchai
Wichai Pornthanakasem
Yongyuth Yuthavong
Darin Kongkasuriyachai
Thailand National Center for Genetic Engineering and Biotechnology
Mahidol University
Keywords: Immunology and Microbiology;Medicine
Issue Date: 1-Jan-2014
Citation: Parasitology International. Vol.63, No.6 (2014), 777-784
Abstract: Malaria is largely a preventable and curable disease. However, a delay or an inappropriate treatment can result in serious adverse outcomes for patient. Rapid, simple and cost-effective diagnostic tests that can be easily adapted and rapidly scaled-up at the field or community levels are needed. In this study, accelerated detection methods for the Plasmodium falciparum (Pf) and Plasmodium vivax (Pv) dihydrofolate reductase-thymidylate synthase were developed based on the loop-mediated isothermal amplification (LAMP) method. The developed methods included the use of species-specific biotinylated primers to amplify LAMP amplicons, which were then hybridized to specific FITC-labeled DNA probes and visualized on a chromatographic lateral flow dipstick (LFD). The total LAMP-LFD assay time was approximately 1.5. h. The LAMP-LFD assays showed similar detection limit to conventional PCR assay when performed on plasmid DNA carrying the malaria dhfr-ts genes. The LAMP-LFD showed 10 folds higher detection limit than PCR when performed on genomic DNA samples from Pf and Pv parasites. The dhfr-ts LAMP-LFD assays also have the advantages of reduced assay time and easy format for interpretation of results. © 2014 Elsevier Ireland Ltd.
URI: https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=84905922588&origin=inward
http://repository.li.mahidol.ac.th/dspace/handle/123456789/34076
ISSN: 18730329
13835769
Appears in Collections:Scopus 2011-2015

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