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Please use this identifier to cite or link to this item: http://repository.li.mahidol.ac.th/dspace/handle/123456789/34248
Title: Effect of synthetic antimicrobial peptides on Naegleria Fowleri trophozoites
Authors: Supathra Tiewcharoen
Watchara Phurttikul
Jundee Rabablert
Prasert Auewarakul
Sittiruk Roytrakul
Pruksawan Chetanachan
Thassanant Atithep
Virach Junnu
Mahidol University
Silpakorn University
Thailand National Center for Genetic Engineering and Biotechnology
National Institutes of Health, Bethesda
Keywords: Medicine
Issue Date: 1-May-2014
Citation: Southeast Asian Journal of Tropical Medicine and Public Health. Vol.45, No.3 (2014), 537-546
Abstract: © 2014, SEAMEO TROPMED Network. All Rights Reserved. We evaluated the effect of tritrpticin, lactoferrin, killer decapeptide and scrambled peptide in vitro against Naegleria fowleri trophozoites compared with amphotericin B. Tritrpticin (100 μg/ml) caused apoptosis of N. fowleri trophozoites (2x105cells/ml), while lactoferrin, killer decapeptide and scrambled peptide did not. On Gormori trichrome staining, tritrpticin affected the elasticity of the surface membrane and reduced the size of the nuclei of N. fowleri trophozoites. The ultrastructure surface membrane and food cup formation of the trophozoites were 100% inhibited. These results are consistent with inhibition of the nfa1, Mp2CL5 of the treated trophozoite, which plays a role in food cup formation. Tritrpticin 100 μg/ml was not toxic against SK-N-MC cells. Our findings suggest tritrpticin has activity against the surface membrane and nfa1and Mp2CL5 of N. fowleri trophozoites and could be developed as a potential therapeutic agent.
URI: https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=84904573634&origin=inward
http://repository.li.mahidol.ac.th/dspace/handle/123456789/34248
ISSN: 01251562
Appears in Collections:Scopus 2011-2015

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