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Please use this identifier to cite or link to this item: http://repository.li.mahidol.ac.th/dspace/handle/123456789/34322
Title: Healthcare-associated outbreak of meticillin-resistant Staphylococcus aureus bacteraemia: Role of a cryptic variant of an epidemic clone
Authors: R. M. Miller
J. R. Price
E. M. Batty
X. Didelot
D. Wyllie
T. Golubchik
D. W. Crook
J. Paul
T. E.A. Peto
D. J. Wilson
M. Cule
C. L.C. Ip
N. P.J. Day
C. E. Moore
R. Bowden
M. J. Llewelyn
Nuffield Department of Clinical Medicine
John Radcliffe Hospital
University of Sussex
University of Oxford
Royal Sussex County Hospital
Wellcome Trust Centre for Human Genetics
Mahidol University
Keywords: Medicine
Issue Date: 14-Jan-2014
Citation: Journal of Hospital Infection. Vol.86, No.2 (2014), 83-89
Abstract: Background: New strains of meticillin-resistant Staphylococcus aureus (MRSA) may be associated with changes in rates of disease or clinical presentation. Conventional typing techniques may not detect new clonal variants that underlie changes in epidemiology or clinical phenotype. Aim: To investigate the role of clonal variants of MRSA in an outbreak of MRSA bacteraemia at a hospital in England. Methods: Bacteraemia isolates of the major UK lineages (EMRSA-15 and -16) from before and after the outbreak were analysed by whole-genome sequencing in the context of epidemiological and clinical data. For comparison, EMRSA-15 and -16 isolates from another hospital in England were sequenced. A clonal variant of EMRSA-16 was identified at the outbreak hospital and a molecular signature test designed to distinguish variant isolates among further EMRSA-16 strains. Findings: By whole-genome sequencing, EMRSA-16 isolates during the outbreak showed strikingly low genetic diversity (P < 1 × 10-6, Monte Carlo test), compared with EMRSA-15 and EMRSA-16 isolates from before the outbreak or the comparator hospital, demonstrating the emergence of a clonal variant. The variant was indistinguishable from the ancestral strain by conventional typing. This clonal variant accounted for 64/72 (89%) of EMRSA-16 bacteraemia isolates at the outbreak hospital from 2006. Conclusions: Evolutionary changes in epidemic MRSA strains not detected by conventional typing may be associated with changes in disease epidemiology. Rapid and affordable technologies for whole-genome sequencing are becoming available with the potential to identify and track the emergence of variants of highly clonal organisms. © 2013 The Authors.
URI: https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=84893688023&origin=inward
http://repository.li.mahidol.ac.th/dspace/handle/123456789/34322
ISSN: 15322939
01956701
Appears in Collections:Scopus 2011-2015

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