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Please use this identifier to cite or link to this item: http://repository.li.mahidol.ac.th/dspace/handle/123456789/34825
Title: High-throughput ultrasensitive molecular techniques for quantifying low-density malaria parasitemias
Authors: Mallika Imwong
Sarun Hanchana
Benoit Malleret
Laurent Rénia
Nicholas P.J. Day
Arjen Dondorp
Francois Nosten
Georges Snounou
Nicholas J. White
Mahidol University
Agency for Science, Technology and Research, Singapore
Yong Loo Lin School of Medicine
Churchill Hospital
Sorbonne Universite
CNRS Centre National de la Recherche Scientifique
Keywords: Medicine
Issue Date: 1-Jan-2014
Citation: Journal of Clinical Microbiology. Vol.52, No.9 (2014), 3303-3309
Abstract: The epidemiology of malaria in "low-transmission" areas has been underestimated. Molecular detection methods have revealed higher prevalences of malaria than conventional microscopy or rapid diagnostic tests, but these typically evaluate finger-prick capillary blood samples (∼5 μl) and therefore cannot detect parasite densities of <200/ml. Their use underestimates true parasite carriage rates. To characterize the epidemiology of malaria in low-transmission settings and plan elimination strategies, more sensitive quantitative PCR (qPCR) is needed to identify and quantify low-density malaria parasitemias. A highly sensitive "high-volume" quantitative PCR (qPCR) method based on Plasmodium sp. 18S RNA was adapted for blood sample volumes of ≥250 μl and scaled for high throughput. The methods were validated by assessment of the analytical sensitivity and specificity, diagnostic sensitivity, and specificity, efficiency, precision, analytical and diagnostic accuracies, limit of detection, root cause analysis of false positives, and robustness. The high-volume qPCR method based on Plasmodium sp. 18S RNA gave high PCR efficiency of 90 to 105%. Concentrations of parasite DNA from large volumes of blood gave a consistent analytical detection limit (LOD) of 22 parasites/ml (95% CI, 21.79 to 74.9), which is some 2,500 times more sensitive than conventional microscopy and 50 times more sensitive than currently used PCR methods from filter paper blood spots. The diagnostic specificity was 99.75%. Using automated procedures it was possible to process 700 blood samples per week. A very sensitive and specific high-throughput high-volume qPCR method for the detection of low-density parasitemias (>20 parasites/ml) was developed and validated. Copyright © 2014 Imwong et al.
URI: https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=84906880712&origin=inward
http://repository.li.mahidol.ac.th/dspace/handle/123456789/34825
ISSN: 1098660X
00951137
Appears in Collections:Scopus 2011-2015

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