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Please use this identifier to cite or link to this item: http://repository.li.mahidol.ac.th/dspace/handle/123456789/34845
Title: Genetic diversity and dynamic distribution of mycobacterium tuberculosis isolates causing pulmonary and extrapulmonary tuberculosis in Thailand
Authors: Prapaporn Srilohasin
Angkana Chaiprasert
Katsushi Tokunaga
Nao Nishida
Therdsak Prammananan
Nat Smittipat
Surakameth Mahasirimongkol
Boonchai Chaiyasirinroje
Hideki Yanai
Prasit Palittapongarnpim
Mahidol University
University of Tokyo
National Center for Global Health and Medicine
Thailand National Center for Genetic Engineering and Biotechnology
Thailand Ministry of Public Health
Research Institute of Tuberculosis
Japan Anti-Tuberculosis Association
Keywords: Medicine
Issue Date: 1-Jan-2014
Citation: Journal of Clinical Microbiology. Vol.52, No.12 (2014), 4267-4274
Abstract: Copyright © 2014, American Society for Microbiology. All Rights Reserved. This study examined the genetic diversity and dynamicity of circulating Mycobacterium tuberculosis strains in Thailand using nearly neutral molecular markers. The single nucleotide polymorphism (SNP)-based genotypes of 1,414 culture-positive M. tuberculosis isolates from 1,282 pulmonary tuberculosis (PTB) and 132 extrapulmonary TB (EPTB) patients collected from 1995 to 2011 were characterized. Among the eight SNP cluster groups (SCG), SCG2 (44.1%), which included the Beijing (BJ) genotype, and SCG1 (39.4%), an East African Indian genotype, were dominant. Comparisons between the genotypes of M. tuberculosis isolates causing PTB and EPTB in HIV-negative cases revealed similar prevalence trends although genetic diversity was higher in the PTB patients. The identification of 10 reported sequence types (STs) and three novel STs was hypothesized to indicate preferential expansion of the SCG2 genotype, especially the modern BJ ST10 (15.6%) and ancestral BJ ST19 (13.1%). An association between SCG2 and SCG1 genotypes and particular patient age groups implies the existence of different genetic advantages among the bacterial populations. The results revealed that increasing numbers of young patients were infected with M. tuberculosis SCGs 2 and 5, which contrasts with the reduction of the SCG1 genotype. Our results indicate the selection and dissemination of potent M. tuberculosis genotypes in this population. The determination of heterogeneity and dynamic population changes of circulating M. tuberculosis strains in countries using the Mycobacterium bovis BCG (bacillus Calmette-Guérin) vaccine are beneficial for vaccine development and control strategies.
URI: https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=84910135618&origin=inward
http://repository.li.mahidol.ac.th/dspace/handle/123456789/34845
ISSN: 1098660X
00951137
Appears in Collections:Scopus 2011-2015

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