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Title: Genetic diversity of Plasmodium falciparum histidine-rich protein 2 in the China-Myanmar border area
Authors: Peipei Li
Hua Xing
Zhenjun Zhao
Zhaoqing Yang
Yaming Cao
Wenli Li
Guiyun Yan
Jetsumon Sattabongkot
Liwang Cui
Qi Fan
Dalian Institute of Biotechnology
Dalian University of Technology
Kunming Medical University
China Medical University Shenyang
University of California, Irvine
Mahidol University
Pennsylvania State University
Keywords: Agricultural and Biological Sciences;Immunology and Microbiology;Medicine
Issue Date: 1-Dec-2015
Citation: Acta Tropica. Vol.152, (2015), 26-31
Abstract: © 2015 Elsevier B.V. Deletion of the Plasmodium falciparum histidine-rich protein 2 (pfhrp2) gene may affect the performance of PfHRP2-based rapid diagnostic tests (RDTs). Here we investigated the genetic diversity of the pfhrp2 gene in clinical parasite isolates collected in recent years from the China-Myanmar border area. Deletion of pfhrp2 has been identified in 4 out of 97 parasite isolates. Sequencing of the pfhrp2 exon2 from 67 isolates revealed a high level of genetic diversity in pfhrp2, which is reflected in the presence of many repeat types and their variants, as well as variable copy numbers and different arrangements of these repeats in parasite isolates. In addition, we observed pfhrp3 deletion in three of the four parasites harboring pfhrp2 deletion, suggesting of double deletions of both genes in these three isolates. Analysis of two cases, which were P. falciparum-positive by microscopy and PCR but failed by two PfHRP2-based RDTs, did not find pfhrp2 deletion. Further correlational studies of pfhrp2 polymorphisms with detection sensitivity are needed to identify factors influencing the performance of RDTs in malaria-endemic areas.
ISSN: 18736254
Appears in Collections:Scopus 2011-2015

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