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dc.contributor.authorWitchuda Payuhakriten_US
dc.contributor.authorTasanee Panichakulen_US
dc.contributor.authorNatthawut Charoenphonen_US
dc.contributor.authorPanus Chalermsaenyakornen_US
dc.contributor.authorAdithep Jaovisidhaen_US
dc.contributor.authorChokdee Wongborisuthen_US
dc.contributor.authorRachanee Udomsangpetchen_US
dc.contributor.otherMahidol Universityen_US
dc.contributor.otherSuan Dusit Universityen_US
dc.contributor.otherFaculty of Medicine, Ramathibodi Hospital, Mahidol Universityen_US
dc.identifier.citationEXCLI Journal. Vol.14, (2015), 1031-1039en_US
dc.description.abstract© 2015, Leibniz Research Centre for Working Environment and Human Factors. All right reserved. Hematopoietic stem cells (HSC) from cord blood are potentially high sources for transplantation due to their low immunogenicity and the presence of the multipotent cells. These cells are capable of differentiating to produce various lineages of blood cells under specific conditions. We have enriched highly purified CD34<sup>+</sup> cells from cord blood, determined in vitro growth of the cells in culture systems in the absence (condition A) or presence of GM-CSF and G-CSF (condition B), and determined the profile of immune cells during the period of cultivation by using flow cytometry. PhytohemagglutininA (PHA) was used as a mitogen to stimulate T lymphocytes derived from hematopoietic stem cells. GM-CSF and G-CSF prolonged the survival of the growing cells and also maintained expansion of cells in blastic stage. By day 12 of cultivation, when cell numbers peaked, various types of immune cells had appeared (CD14<sup>+</sup> cells, CD40<sup>+</sup>HLA-DR<sup>+</sup> cells, CD3<sup>+</sup>CD56<sup>+</sup> cells, CD19<sup>+</sup> cells, CD3<sup>+</sup>CD4<sup>+</sup> cells, CD3<sup>+</sup>CD8<sup>+</sup>cells and CD3-CD56<sup>+</sup>). A significantly higher percentage of monocytes (p = 0.002) were observed under culture with GM-CSF, G-CSF when compared with culture without GM-CSF, G-CSF. In addition, T lymphocytes derived from HSC responded to 50 μg/ml of PHA. This is the first report showing the complete differentiation and proliferation of immune cells derived from CD34<sup>+</sup> HSC under in vitro culture conditions. Lymphocytes, monocytes, dendritic cells and polymorph nuclear cells derived from HSC in vitro are unique, and thus may benefit various studies such as innate immunity and pathophysiology of immune disorders.en_US
dc.rightsMahidol Universityen_US
dc.subjectAgricultural and Biological Sciencesen_US
dc.subjectBiochemistry, Genetics and Molecular Biologyen_US
dc.titleIn vitro production of functional immune cells derived from human hematopoietic stem cellsen_US
Appears in Collections:Scopus 2011-2015

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