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Title: | Enhancement of shrimp immunity against white spot syndrome virus by Macrobrachium rosenbergii nodavirus-like particle encapsulated VP28 double-stranded RNA |
Authors: | Pitchanee Jariyapong Wattana Weerachatyanukul Sataporn Direkbusarakom Ikuo Hirono Suwit Wuthisuthimethavee Charoonroj Chotwiwatthanakun Walailak University Mahidol University National University Corporation Tokyo University of Marine Science and Technology |
Keywords: | Agricultural and Biological Sciences |
Issue Date: | 1-Sep-2015 |
Citation: | Aquaculture. Vol.446, (2015), 325-332 |
Abstract: | © 2015 Elsevier B.V. We investigated the efficiency and downstream effects of Macrobrachium rosenbergii nodavirus-like particle (MrNv-VLP) encapsulated VP28 double-stranded RNA against white spot syndrome virus (WSSV) in shrimp. Our results showed that the VP28 gene of WSSV was significantly silenced at 72. h post-viral challenge in group pre-treated with the VLP encapsulated VP28 dsRNA. At 24. h post-treatment, the amount of VP28 dsRNA was significantly higher (two-fold) in encapsulated VP28 dsRNA-VLP pre-treated shrimp compared with non-encapsulated VP28 dsRNA pre-treated shrimp. Using quantitative real time polymerase chain reaction (PCR), we found higher and longer expression of RNA-induced silencing complex (RISC) and immune-related genes in shrimp pre-treated with encapsulated VP28 dsRNA compared to the control groups. Increasing expression of apoptotic-related genes was also associated with the encapsulated VP28 dsRNA-VLP pretreatment group at 24. h post-WSSV challenge. These results indicate that MrNv-VLP was able to efficiently deliver VP28 dsRNA into shrimp tissues, which in turn, triggered a better anti-viral response. This may represent a novel strategy for aquaculture disease management. Statement of relevance: This study developed methods for WSSV protection. |
URI: | https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=84930226294&origin=inward http://repository.li.mahidol.ac.th/dspace/handle/123456789/35107 |
ISSN: | 00448486 |
Appears in Collections: | Scopus 2011-2015 |
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