Simple jQuery Dropdowns
Please use this identifier to cite or link to this item: http://repository.li.mahidol.ac.th/dspace/handle/123456789/35107
Title: Enhancement of shrimp immunity against white spot syndrome virus by Macrobrachium rosenbergii nodavirus-like particle encapsulated VP28 double-stranded RNA
Authors: Pitchanee Jariyapong
Wattana Weerachatyanukul
Sataporn Direkbusarakom
Ikuo Hirono
Suwit Wuthisuthimethavee
Charoonroj Chotwiwatthanakun
Walailak University
Mahidol University
National University Corporation Tokyo University of Marine Science and Technology
Keywords: Agricultural and Biological Sciences
Issue Date: 1-Sep-2015
Citation: Aquaculture. Vol.446, (2015), 325-332
Abstract: © 2015 Elsevier B.V. We investigated the efficiency and downstream effects of Macrobrachium rosenbergii nodavirus-like particle (MrNv-VLP) encapsulated VP28 double-stranded RNA against white spot syndrome virus (WSSV) in shrimp. Our results showed that the VP28 gene of WSSV was significantly silenced at 72. h post-viral challenge in group pre-treated with the VLP encapsulated VP28 dsRNA. At 24. h post-treatment, the amount of VP28 dsRNA was significantly higher (two-fold) in encapsulated VP28 dsRNA-VLP pre-treated shrimp compared with non-encapsulated VP28 dsRNA pre-treated shrimp. Using quantitative real time polymerase chain reaction (PCR), we found higher and longer expression of RNA-induced silencing complex (RISC) and immune-related genes in shrimp pre-treated with encapsulated VP28 dsRNA compared to the control groups. Increasing expression of apoptotic-related genes was also associated with the encapsulated VP28 dsRNA-VLP pretreatment group at 24. h post-WSSV challenge. These results indicate that MrNv-VLP was able to efficiently deliver VP28 dsRNA into shrimp tissues, which in turn, triggered a better anti-viral response. This may represent a novel strategy for aquaculture disease management. Statement of relevance: This study developed methods for WSSV protection.
URI: https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=84930226294&origin=inward
http://repository.li.mahidol.ac.th/dspace/handle/123456789/35107
ISSN: 00448486
Appears in Collections:Scopus 2011-2015

Files in This Item:
There are no files associated with this item.


Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.