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|Title:||Enhancement of shrimp immunity against white spot syndrome virus by Macrobrachium rosenbergii nodavirus-like particle encapsulated VP28 double-stranded RNA|
National University Corporation Tokyo University of Marine Science and Technology
|Keywords:||Agricultural and Biological Sciences|
|Citation:||Aquaculture. Vol.446, (2015), 325-332|
|Abstract:||© 2015 Elsevier B.V. We investigated the efficiency and downstream effects of Macrobrachium rosenbergii nodavirus-like particle (MrNv-VLP) encapsulated VP28 double-stranded RNA against white spot syndrome virus (WSSV) in shrimp. Our results showed that the VP28 gene of WSSV was significantly silenced at 72. h post-viral challenge in group pre-treated with the VLP encapsulated VP28 dsRNA. At 24. h post-treatment, the amount of VP28 dsRNA was significantly higher (two-fold) in encapsulated VP28 dsRNA-VLP pre-treated shrimp compared with non-encapsulated VP28 dsRNA pre-treated shrimp. Using quantitative real time polymerase chain reaction (PCR), we found higher and longer expression of RNA-induced silencing complex (RISC) and immune-related genes in shrimp pre-treated with encapsulated VP28 dsRNA compared to the control groups. Increasing expression of apoptotic-related genes was also associated with the encapsulated VP28 dsRNA-VLP pretreatment group at 24. h post-WSSV challenge. These results indicate that MrNv-VLP was able to efficiently deliver VP28 dsRNA into shrimp tissues, which in turn, triggered a better anti-viral response. This may represent a novel strategy for aquaculture disease management. Statement of relevance: This study developed methods for WSSV protection.|
|Appears in Collections:||Scopus 2011-2015|
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