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Please use this identifier to cite or link to this item: http://repository.li.mahidol.ac.th/dspace/handle/123456789/35116
Title: Molecular evolution of PvMSP3a block II in Plasmodium vivax from diverse geographic origins
Authors: Bhavna Gupta
B. P.Niranjan Reddy
Qi Fan
Guiyun Yan
Jeeraphat Sirichaisinthop
Jetsumon Sattabongkot
Ananias A. Escalante
Liwang Cui
Luzia Helena Carvalho
Pennsylvania State University
Dalian Institute of Biotechnology
University of California, Irvine
Vector Borne Disease Training Center
Mahidol University
Temple University
Fundacao Oswaldo Cruz
Keywords: Agricultural and Biological Sciences;Biochemistry, Genetics and Molecular Biology
Issue Date: 12-Aug-2015
Citation: PLoS ONE. Vol.10, No.8 (2015)
Abstract: © 2015 Gupta et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. Block II of Plasmodium vivax merozoite surface protein 3α (PvMSP3α) is conserved and has been proposed as a potential candidate for a malaria vaccine. The present study aimed to compare sequence diversity in PvMSP3a block II at a local microgeographic scale in a village as well as from larger geographic regions (countries and worldwide). Blood samples were collected from asymptomatic carriers of P. vivax in a village at the western border of Thailand and PvMSP3α was amplified and sequenced. For population genetic analysis, 237 PvMSP3α block II sequences from eleven P. vivax endemic countries were analyzed. PvMSP3α sequences from 20 village-level samples revealed two length variant types with one type containing a large deletion in block I. In contrast, block II was relatively conserved; especially, some non-synonymous mutations were extensively shared among 11 parasite populations. However, the majority of the low-frequency synonymous variations were population specific. The conserved pattern of nucleotide diversity in block II sequences was probably due to functional/structural constraints, which were further supported by the tests of neutrality. Notably, a small region in block II that encodes a predicted B cell epitope was highly polymorphic and showed signs of balancing selection, signifying that this region might be influenced by the immune selection and may serve as a starting point for designing multi-antigen/stage epitope based vaccines against this parasite.
URI: https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=84943172094&origin=inward
http://repository.li.mahidol.ac.th/dspace/handle/123456789/35116
ISSN: 19326203
Appears in Collections:Scopus 2011-2015

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