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Please use this identifier to cite or link to this item: http://repository.li.mahidol.ac.th/dspace/handle/123456789/35117
Title: The immunoreactive exo-1,3-β-glucanase from the pathogenic oomycete pythium insidiosum is temperature regulated and exhibits glycoside hydrolase activity
Authors: Angsana Keeratijarut
Tassanee Lohnoo
Thidarat Rujirawat
Wanta Yingyong
Thareerat Kalambaheti
Shannon Miller
Vipaporn Phuntumart
Theerapong Krajaejun
Mahidol University
Bowling Green State University
Keywords: Agricultural and Biological Sciences;Biochemistry, Genetics and Molecular Biology
Issue Date: 11-Aug-2015
Citation: PLoS ONE. Vol.10, No.8 (2015)
Abstract: © 2015 Keeratijarut et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. The oomycete organism, Pythium insidiosum, is the etiologic agent of the life-threatening infectious disease called "pythiosis". Diagnosis and treatment of pythiosis is difficult and challenging. Novel methods for early diagnosis and effective treatment are urgently needed. Recently, we reported a 74-kDa immunodominant protein of P. insidiosum, which could be a diagnostic target, vaccine candidate, and virulence factor. The protein was identified as a putative exo-1,3-ß-glucanase (Exo1). This study reports on genetic, immunological, and biochemical characteristics of Exo1. The full-length exo1 coding sequence (2,229 bases) was cloned. Phylogenetic analysis showed that exo1 is grouped with glucanase-encoding genes of other oomycetes, and is far different from glucanase-encoding genes of fungi. exo1 was up-regulated upon exposure to body temperature, and its gene product is predicted to contain BglC and X8 domains, which are involved in carbohydrate transport, binding, and metabolism. Based on its sequence, Exo1 belongs to the Glycoside Hydrolase family 5 (GH5). Exo1, expressed in E. coli, exhibited ß-glucanase and cellulase activities. Exo1 is a major intracellular immunoreactive protein that can trigger host immune responses during infection. Since GH5 enzyme-encoding genes are not present in human genomes, Exo1 could be a useful target for drug and vaccine development against this pathogen.
URI: https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=84942852380&origin=inward
http://repository.li.mahidol.ac.th/dspace/handle/123456789/35117
ISSN: 19326203
Appears in Collections:Scopus 2011-2015

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