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Title: Interaction study of a novel Macrobrachium rosenbergii effector caspase with B2 and capsid proteins of M.rosenbergii nodavirus reveals their roles in apoptosis
Authors: Supak Youngcharoen
Saengchan Senapin
Tareerat Lertwimol
Siwaporn Longyant
Paisarn Sithigorngul
Timothy W. Flegel
Parin Chaivisuthangkura
Srinakharinwirot University
Mahidol University
Thailand National Center for Genetic Engineering and Biotechnology
Thailand National Metal and Materials Technology Center
Keywords: Agricultural and Biological Sciences;Environmental Science;Medicine
Issue Date: 1-Aug-2015
Citation: Fish and Shellfish Immunology. Vol.45, No.2 (2015), 534-542
Abstract: © 2015 Elsevier Ltd. Apoptosis is an essential immune response to protect invertebrates from virus infected cells. In shrimp, virus infection has been reported to induce apoptosis. Macrobrachium rosenbergii (. Mr) was considered to be a disease-resistant host when compared to penaeid shrimps. Caspase-3 was classified as an executioner caspase which played a key role in virus-induced apoptosis. In this study, an effector caspase gene of M.rosenbergii (. Mrcasp) was cloned and characterized. The open reading frame (ORF) of Mrcasp was 957 nucleotide encoding 318 amino acid with a deduced molecular mass of 35.87kDa. RT-PCR analysis showed the presence of Mrcasp in all examined tissues. The phylogenetic tree indicated that Mrcasp was closely related with caspase 3 of shrimp. The functions of the Mrcasp, B2 and capsid proteins of M. rosenbergii nodavirus (. MrNV) were assayed in Sf-9 cells. The results showed that Mrcasp induce apoptotic morphology cells; however, capsid protein of MrNV could inhibit apoptotic cells whereas B2 could neither induce nor inhibit apoptotic cells by DAPI staining. The protein interaction between Mrcasp and viral MrNV structure revealed that Mrcasp did not bind to B2 or capsid protein whereas B2 and capsid proteins could bind directly to each other. This study reported a novel sequence of a full-length Mrcasp and its functional studies indicated that Mrcasp could induce apoptotic cells. Our data is the first report demonstrating the direct protein-protein interaction between capsid protein and B2 protein of MrNV.
ISSN: 10959947
Appears in Collections:Scopus 2011-2015

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