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Title: Molecular cloning and characterization of Mj-mov-10, a putative RNA helicase involved in RNAi of kuruma shrimp
Authors: Amnat Phetrungnapha
Hidehiro Kondo
Ikuo Hirono
Sakol Panyim
Chalermporn Ongvarrasopone
Naresuan University
National University Corporation Tokyo University of Marine Science and Technology
Mahidol University
Keywords: Agricultural and Biological Sciences;Environmental Science
Issue Date: 1-May-2015
Citation: Fish and Shellfish Immunology. Vol.44, No.1 (2015), 241-247
Abstract: © 2015 Elsevier Ltd. Identification and characterization of the RNAi-related genes is the key to understanding RNAi mechanism in shrimp. In this study, we have identified and characterized a novel putative RNA helicase gene, Mj-mov-10 from the kuruma shrimp, Marsupenaeus japonicus and its implication in shrimp RNAi was demonstrated. The full-length Mj-mov-10 gene contained 3536. bp, including 239bp of 5'UTR, 2895bp of the open reading frame (ORF) and 402. bp of 3'UTR, respectively. An ORF of Mj-mov-10 could be translated to a 109-kDa protein which consists of a single helicase core domain containing seven signature motifs of the RNA helicase superfamily-1. Mj-MOV-10 protein shared 47% and 40% identity with mammalian MOV-10 and plant SDE3, respectively. Expression of Mj-mov-10 gene was significantly up-regulated upon dsRNA and white spot syndrome virus (WSSV) challenge. Invivo gene knockdown of Mj-mov-10 resulted in an increase of a susceptibility of shrimp to WSSV infection. Our results implied the functional significance of Mj-MOV-10 in dsRNA-mediated gene silencing and antiviral defense mechanism in shrimp.
ISSN: 10959947
Appears in Collections:Scopus 2011-2015

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