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Title: Development of an in vitro assay and demonstration of Plasmodium berghei liver- Stage inhibition by TRAP-specific CD8+ T Cells
Authors: Rhea J. Longley
Karolis Bauza
Katie J. Ewer
Adrian V.S. Hill
Alexandra J. Spencer
University of Oxford
Walter and Eliza Hall Institute of Medical Research
Mahidol University
University of Melbourne
Keywords: Agricultural and Biological Sciences;Biochemistry, Genetics and Molecular Biology
Issue Date: 30-Mar-2015
Citation: PLoS ONE. Vol.10, No.3 (2015)
Abstract: © 2015 Longley et al. The development of an efficacious vaccine against the Plasmodium parasite remains a top priority. Previous research has demonstrated the ability of a prime-boost virally vectored sub-unit vaccination regimen, delivering the liver-stage expressed malaria antigen TRAP, to produce high levels of antigen-specific T cells. The liver-stage of malaria is the main target of T cell-mediated immunity, yet a major challenge in assessing new T cell inducing vaccines has been the lack of a suitable pre-clinical assay. We have developed a flow-cytometry based in vitro T cell killing assay using a mouse hepatoma cell line, Hepa1-6, and Plasmodium berghei GFP expressing sporozoites. Using this assay, P. berghei TRAP-specific CD8+T cell enriched splenocytes were shown to inhibit liver-stage parasites in an ef-fector- to-target ratio dependent manner. Further development of this assay using human hepatocytes and P. falciparum would provide a new method to pre-clinically screen vaccine candidates and to elucidate mechanisms of protection in vitro.
ISSN: 19326203
Appears in Collections:Scopus 2011-2015

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