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Title: Development and validation of LC-MS/MS method for quantitative determination of adenosine, guanosine, xanthine and uric acid in widely consumed vegetables in Thailand
Authors: Narisa Rukdee
Piyanuch Rojsanga
Chutima Matayatsuk Phechkrajang
Mahidol University
Keywords: Agricultural and Biological Sciences;Medicine
Issue Date: 1-Jan-2015
Citation: Natural Product Communications. Vol.10, No.8 (2015), 1435-1437
Abstract: In this study, a triple quadrupole LC-MS/MS electrospray ionization method was developed and validated for quantitative determination of adenosine, guanosine, xanthine and uric acid in fifteen widely consumed Thai vegetables. The method was successively developed by using caffeine as internal standard. The lower limit of quantitation (LLOQ) was 0.2 μg/g for adenosine and guanosine, and 1.0 μg/g for uric acid and xanthine. The method was fully validated according to USFDA guidelines and all performance characteristics were found acceptable. Subsequently, the developed and validated LC-MS/MS method was applied to determine the four interest substances in fifteen widely consumed vegetables in Thailand. The results showed that all vegetables included in the study could be classified as low adenosine, guanosine, xanthine and uric acid containing foods since the concentrations of these substances were less than 50 mg per 100 g. This finding was enormously valuable information for hyperuricemia and gouty patients.
ISSN: 15559475
Appears in Collections:Scopus 2011-2015

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