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Title: Lectin-based profiling of coelomocytes in holothuria scabra and expression of superoxide dismutase in purified coelomocytes
Authors: Yanvit Prompoon
Wattana Weerachatyanukul
Boonsirm Withyachumnarnkul
Rapeepun Vanichviriyakit
Kanokpan Wongprasert
Somluk Asuvapongpatana
Mahidol University
Nakhon Sri Thammarat
Keywords: Agricultural and Biological Sciences
Issue Date: 1-Jan-2015
Citation: Zoological Science. Vol.32, No.4 (2015), 345-351
Abstract: © 2015 Zoological Society of Japan. Coelomocytes are the first line of immune defense in marine animals. Their distributions are greatly variable even in the close animal species. In this study, we used lectin staining to aid in the classification and purification of these cells for further investigation of SOD distribution among coelomocytes of H. scraba. We classified coelomocytes into four types: type 1, lymphocytes; type 2, phagocytes; type 3, spherulocytes; and type 4, giant cells. Among four lectins used, Con A appeared to give a broad reactivity against most coelomocytes, except for giant cells. In addition, phagocytes usually engaged the highest fluorescent intensity with most lectins, with the exception of PNA, for which spherulocytes possessed the highest fluorescent intensity. Using FACS for fraction collection, it was found that F1 fraction contained the purest phagocyte population (> 95%), which was highly reactive with anti- superoxide dismutase (SOD) as revealed by immunoblotting and immunofluorescence staining, although some minor staining was also detected in spherulocytes. Our results thus provide a fundamental platform for comparing alterations that may happen to the population and SOD contents of coelomocytes when the sea cucumber is subjected to environmental changes that would activate their immune responses.
ISSN: 02890003
Appears in Collections:Scopus 2011-2015

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