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Title: | Delivery of double stranded RNA by Macrobrachium rosenbergii nodavirus-like particles to protect shrimp from white spot syndrome virus |
Authors: | Pitchanee Jariyapong Charoonroj Chotwiwatthanakun Sataporn Direkbusarakom Ikuo Hirono Suwit Wuthisuthimethavee Wattana Weerachatyanukul Walailak University Mahidol University National University Corporation Tokyo University of Marine Science and Technology |
Keywords: | Agricultural and Biological Sciences |
Issue Date: | 1-Jan-2015 |
Citation: | Aquaculture. Vol.435, (2015), 86-91 |
Abstract: | © 2014 Elsevier B.V. We tested the use of Macrobrachium rosenbergii nodavirus-like particles (MrNv-VLPs) as a delivery mechanism to carry therapeutic agents against white spot syndrome in shrimp. We used constructed double-stranded RNA called VP28 (VP28 dsRNA) against WSSV envelope genes to confer protection against the pathogen. Results showed that MrNv-VLP was able to encapsulate VP28 dsRNA. Using enhanced green fluorescent protein (EGFP) as a reporter, we found that VLP penetrated various shrimp tissues including the muscle, hepatopancreas, and gill. A statistically significant relative survival rate of 44.5% was obtained in the group of shrimp receiving encapsulated VP28 dsRNA-VLP after WSSV challenge as compared to 100% mortality in the control shrimp at 7. days post-infection. Shrimp treated with EGFP dsRNA loaded into MrNv-VLPs showed relatively similar motility rates as those of controls. Moreover, MrNv-VLP encapsulation improved VP28 dsRNA efficiency against WSSV. A higher survival rate of 16.7% was observed in the group of shrimp receiving encapsulated VP28 dsRNA-VLP when compared to those receiving naked VP28 dsRNA. These results indicate that MrNv-VLP is a good candidate for use as a therapeutic delivery system against shrimp diseases due to its self-reassembly property, broad target of various shrimp tissues and immune enhancement. |
URI: | https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=84908431508&origin=inward http://repository.li.mahidol.ac.th/dspace/handle/123456789/35240 |
ISSN: | 00448486 |
Appears in Collections: | Scopus 2011-2015 |
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