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dc.contributor.authorPitchanee Jariyapongen_US
dc.contributor.authorCharoonroj Chotwiwatthanakunen_US
dc.contributor.authorSataporn Direkbusarakomen_US
dc.contributor.authorIkuo Hironoen_US
dc.contributor.authorSuwit Wuthisuthimethaveeen_US
dc.contributor.authorWattana Weerachatyanukulen_US
dc.contributor.otherWalailak Universityen_US
dc.contributor.otherMahidol Universityen_US
dc.contributor.otherNational University Corporation Tokyo University of Marine Science and Technologyen_US
dc.date.accessioned2018-11-23T09:33:24Z-
dc.date.available2018-11-23T09:33:24Z-
dc.date.issued2015-01-01en_US
dc.identifier.citationAquaculture. Vol.435, (2015), 86-91en_US
dc.identifier.issn00448486en_US
dc.identifier.other2-s2.0-84908431508en_US
dc.identifier.urihttps://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=84908431508&origin=inwarden_US
dc.identifier.urihttp://repository.li.mahidol.ac.th/dspace/handle/123456789/35240-
dc.description.abstract© 2014 Elsevier B.V. We tested the use of Macrobrachium rosenbergii nodavirus-like particles (MrNv-VLPs) as a delivery mechanism to carry therapeutic agents against white spot syndrome in shrimp. We used constructed double-stranded RNA called VP28 (VP28 dsRNA) against WSSV envelope genes to confer protection against the pathogen. Results showed that MrNv-VLP was able to encapsulate VP28 dsRNA. Using enhanced green fluorescent protein (EGFP) as a reporter, we found that VLP penetrated various shrimp tissues including the muscle, hepatopancreas, and gill. A statistically significant relative survival rate of 44.5% was obtained in the group of shrimp receiving encapsulated VP28 dsRNA-VLP after WSSV challenge as compared to 100% mortality in the control shrimp at 7. days post-infection. Shrimp treated with EGFP dsRNA loaded into MrNv-VLPs showed relatively similar motility rates as those of controls. Moreover, MrNv-VLP encapsulation improved VP28 dsRNA efficiency against WSSV. A higher survival rate of 16.7% was observed in the group of shrimp receiving encapsulated VP28 dsRNA-VLP when compared to those receiving naked VP28 dsRNA. These results indicate that MrNv-VLP is a good candidate for use as a therapeutic delivery system against shrimp diseases due to its self-reassembly property, broad target of various shrimp tissues and immune enhancement.en_US
dc.rightsMahidol Universityen_US
dc.source.urihttps://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=84908431508&origin=inwarden_US
dc.subjectAgricultural and Biological Sciencesen_US
dc.titleDelivery of double stranded RNA by Macrobrachium rosenbergii nodavirus-like particles to protect shrimp from white spot syndrome virusen_US
dc.typeArticleen_US
dc.rights.holderSCOPUSen_US
dc.identifier.doi10.1016/j.aquaculture.2014.09.034en_US
Appears in Collections:Scopus 2011-2015

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