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|Title:||Identification of Differentially Expressed Proteins in Cassava Infected with Colletotrichum gloeosporioides f. sp. manihotis|
Duncan R. Smith
Thailand Ministry of Agriculture and Cooperatives
Thailand National Center for Genetic Engineering and Biotechnology
|Keywords:||Agricultural and Biological Sciences;Biochemistry, Genetics and Molecular Biology|
|Citation:||Journal of Crop Improvement. Vol.29, No.6 (2015), 728-746|
|Abstract:||Copyright © Taylor & Francis Group, LLC. Cassava anthracnose disease (CAD), caused by Colletotrichum gloeosporioides f. sp. manihotis infection, is a major disease of cassava (Manihot esculenta Crantz). The objective of this study was to identify proteins differentially regulated in resistant (‘Huaybong 60’) and susceptible (‘Hanatee’) cultivars in response to C. gloeosporioides f. sp. manihotis infection. Total proteins were extracted and resolved via 2-dimensional gel electrophoresis. Fourteen differentially expressed proteins were identified in ‘Huaybong 60’, of which eight were up-regulated and seven down-regulated. In ‘Hanatee’, seven proteins were identified, of which one was up-regulated and six were down-regulated. Proteins associated with carbohydrate metabolism were down-regulated, whereas reactive oxygen species-generating and cyanogenic pathways were up-regulated in the resistant cultivar. This study provided new insights into the mechanisms underlying pathogen resistance in cassava and suggested that pathogen resistance might arise from an integrated mechanism that arises from only a few initiating events.|
|Appears in Collections:||Scopus 2011-2015|
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