Simple jQuery Dropdowns
Please use this identifier to cite or link to this item: http://repository.li.mahidol.ac.th/dspace/handle/123456789/35284
Title: Microparticles provide a novel biomarker to predict severe clinical outcomes of dengue virus infection
Authors: Nuntaya Punyadee
Dumrong Mairiang
Somchai Thiemmeca
Chulaluk Komoltri
Wirichada Pan-ngum
Nusara Chomanee
Komgrid Charngkaew
Nattaya Tangthawornchaikul
Wannee Limpitikul
Sirijitt Vasanawathana
Prida Malasit
Panisadee Avirutnan
Mahidol University
Thailand National Center for Genetic Engineering and Biotechnology
Songkhla Hospital
Khon Kaen Regional Hospital
Keywords: Agricultural and Biological Sciences;Immunology and Microbiology
Issue Date: 1-Jan-2015
Citation: Journal of Virology. Vol.89, No.3 (2015), 1587-1607
Abstract: © 2015, American Society for Microbiology. Shedding of microparticles (MPs) is a consequence of apoptotic cell death and cellular activation. Low levels of circulating MPs in blood help maintain homeostasis, whereas increased MP generation is linked to many pathological conditions. Herein, we investigated the role of MPs in dengue virus (DENV) infection. Infection of various susceptible cells by DENV led to apoptotic death and MP release. These MPs harbored a viral envelope protein and a nonstructural protein 1 (NS1) on their surfaces. Ex vivo analysis of clinical specimens from patients with infections of different degrees of severity at multiple time points revealed that MPs generated from erythrocytes and platelets are two major MP populations in the circulation of DENV-infected patients. Elevated levels of red blood cell-derived MPs (RMPs) directly correlated with DENV disease severity, whereas a significant decrease in platelet-derived MPs was associated with a bleeding tendency. Removal by mononuclear cells of complement-opsonized NS1-anti-NS1 immune complexes bound to erythrocytes via complement receptor type 1 triggered MP shedding in vitro, a process that could explain the increased levels of RMPs in severe dengue. These findings point to the multiple roles of MPs in dengue pathogenesis. They offer a potential novel biomarker candidate capable of differentiating dengue fever from the more serious dengue hemorrhagic fever.
URI: https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=84921466925&origin=inward
http://repository.li.mahidol.ac.th/dspace/handle/123456789/35284
ISSN: 10985514
0022538X
Appears in Collections:Scopus 2011-2015

Files in This Item:
There are no files associated with this item.


Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.