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Title: Ultrasensitive electrochemical immunosensor based on dual signal amplification process for p16<sup>INK4a</sup> cervical cancer detection in clinical samples
Authors: Pattasuda Duangkaew
Satita Tapaneeyakorn
Chayachon Apiwat
Tararaj Dharakul
Somsak Laiwejpithaya
Proespichaya Kanatharana
Rawiwan Laocharoensuk
Thailand National Science and Technology Development Agency
Mahidol University
Prince of Songkla University
Keywords: Biochemistry, Genetics and Molecular Biology;Chemistry;Engineering
Issue Date: 5-Dec-2015
Citation: Biosensors and Bioelectronics. Vol.74, (2015), 673-679
Abstract: © 2015 Elsevier B.V.. The p16<sup>INK4a</sup> (p16) is a cyclin-dependent kinase inhibitor, which has been evaluated in several studies as a diagnostic marker of cervical cancer. Immunostaining using p16 specific antibody has confirmed an over-expression of p16 protein in cervical cancer cells and its association with disease progression. This article reports an ultrasensitive electrochemical immunosensor for specific detection of p16 and demonstrates its performance for detection of solubilized p16 protein in cell lysates obtained from patients. Sandwich-based immunoreaction couple with double signal amplification strategy based on catalytic enlargement of particle tag was used for high sensitivity and specificity. The conditions were optimized to create an immunoassay protocol. Disposable screen-printed electrode modified with capture antibodies (Ab<inf>1</inf>) was selected for further implementation towards point-of-care diagnostics. Small gold nanoparticles (15nm diameter) conjugated with detection antibodies (Ab<inf>2</inf>) were found to better serve as a detection label due to limited interference with antigen-antibody interaction. Double signal enhancement was performed by sequential depositions of gold and silver layers. This gave the sensitivity of 1.78μAmL(ng GST-p16)<sup>-1</sup>cm<sup>-2</sup> and detection limit of 1.3ngmL<sup>-1</sup> for GST-p16 protein which is equivalent to 0.49ngmL<sup>-1</sup> for p16 protein and 28 cells for HeLa cervical cancer cells. In addition to purified protein, the proposed immunosensor effectively detected elevated p16 level in cervical swab samples obtained from 10 patients with positive result from standard Pap smear test, indicating that an electrochemical immunosensors hold an excellent promise for detection of cervical cancer in clinical setting.
ISSN: 18734235
Appears in Collections:Scopus 2011-2015

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