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Title: SPR-DNA array for detection of methicillin-resistant Staphylococcus aureus (MRSA) in combination with loop-mediated isothermal amplification
Authors: Kawin Nawattanapaiboon
Wansika Kiatpathomchai
Pitak Santanirand
Apirom Vongsakulyanon
Ratthasart Amarit
Armote Somboonkaew
Boonsong Sutapun
Toemsak Srikhirin
Mahidol University
Thailand National Center for Genetic Engineering and Biotechnology
Thailand National Electronics and Computer Technology Center
Suranaree University of Technology
Keywords: Biochemistry, Genetics and Molecular Biology;Chemistry;Engineering;Medicine
Issue Date: 5-Dec-2015
Citation: Biosensors and Bioelectronics. Vol.74, (2015), 335-340
Abstract: © 2015 Elsevier B.V.. In this study, we evaluated surface plasmon resonance imaging (SPR imaging) as a DNA biosensor for the detection of methicillin-resistant Staphylococcus aureus (MRSA) which is one of the most common causes of nosocomial infections. The DNA sample were collected from clinical specimens, including sputum and blood hemoculture were undergone LAMP amplification for 0.18. kbp and 0.23. kbp DNA fragments of femB and mecA genes, respectively. The self-assembled monolayer surface (SAMs) was used for immobilized streptavidin-biotinylated probes on the sensor surface for the detection of LAMP amplicons from MRSA. Both LAMP amplicons were simultaneously hybridized with ssDNA probes immobilized onto a bio-functionalized surface to detect specific targets in the multiplex DNA array platform. In addition, the sensor surface could be regenerated allowing at least five cycles of use with a shortened assay time. The detection limit of LAMP-SPR sensing was 10 copies/μl and LAMP-SPR sensing system showed a good selectivity toward the MRSA.
ISSN: 18734235
Appears in Collections:Scopus 2011-2015

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