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Title: Cytotoxic effect and mechanism inducing cell death of α-mangostin liposomes in various human carcinoma and normal cells
Authors: Ruthairat Benjakul
Lalana Kongkaneramit
Narong Sarisuta
Primchanien Moongkarndi
Christel C. Müller-Goymann
Mahidol University
Srinakharinwirot University
Thammasat University
Technische Universitat Braunschweig
Keywords: Biochemistry, Genetics and Molecular Biology;Medicine
Issue Date: 8-Aug-2015
Citation: Anti-Cancer Drugs. Vol.26, No.8 (2015), 824-834
Abstract: Copyright © 2015 Wolters Kluwer Health, Inc. All rights reserved. The aims of this study were to develop α-mangostin liposomes as well as to evaluate their physicochemical properties and cytotoxic activity. α-Mangostin liposomes were prepared using the reverse-phase evaporation method with lipid composition of phosphatidylcholine to cholesterol at 7: 3 molar ratios; their physicochemical properties and antiproliferative activity were assessed using an MTT assay in four human carcinoma cells [that is, human lung epithelial carcinoma (Calu-3), human colon carcinoma (HT-29), human breast carcinoma (MCF-7), and human colon carcinoma (Caco-2) cells], and two human normal cells [that is, human dermal fibroblasts (HDF) and human adult low-calcium elevated temperature (HaCaT) keratinocytes]. Determinations of morphological changes and oligonucleosomal DNA fragments were also carried out. The liposomal dispersions obtained were unilamellar vesicles as confirmed by cryotransmission and freeze-fracture electron microscopy with a particle size of 114 nm and a ζ potential of -2.56 mV. The <sup>31</sup>P-NMR spectra showed that α-mangostin molecules orientated in the phospholipid bilayer membrane. The α-mangostin could appreciably be entrapped with an efficiency and loading of 81 and 4%, respectively. The antiproliferative activity of α-mangostin liposomes in various cancer and normal cells showed a dose-dependent inhibition in all treated cell lines. The antiproliferative effect of α-mangostin liposomes was found to be associated with apoptosis, with differences in sensitivity among the cell lines treated.
ISSN: 14735741
Appears in Collections:Scopus 2011-2015

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