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|Title:||Construction of CoA-dependent 1-butanol synthetic pathway functions under aerobic conditions in Escherichia coli|
Alisa S. Vangnai
|Keywords:||Biochemistry, Genetics and Molecular Biology;Chemical Engineering;Immunology and Microbiology|
|Citation:||Journal of Biotechnology. Vol.204, (2015), 25-32|
|Abstract:||© 2015 Elsevier B.V. 1-Butanol is an important industrial platform chemical and an advanced biofuel. While various groups have attempted to construct synthetic pathways for 1-butanol production, efforts to construct a pathway that functions under aerobic conditions have met with limited success. Here, we constructed a CoA-dependent 1-butanol synthetic pathway that functions under aerobic conditions in Escherichia coli, by expanding the previously reported (R)-1,3-butanediol synthetic pathway. The pathway consists of phaA (acetyltransferase) and phaB (NADPH-dependent acetoacetyl-CoA reductase) from Ralstonia eutropha, phaJ ((R)-specific enoyl-CoA hydratase) from Aeromonas caviae, ter (trans-enoyl-CoA reductase) from Treponema denticola, bld (butylraldehyde dehydrogenase) from Clostridium saccharoperbutylacetonicum, and inherent alcohol dehydrogenase(s) from E. coli. To evaluate the potential of this pathway for 1-butanol production, culture conditions, including volumetric oxygen transfer coefficient (k<inf>L</inf>a) and pH were optimized in a mini-jar fermenter. Under optimal conditions, 1-butanol was produced at a concentration of up to 8.60 gL<sup>-1</sup> after 46h of fed-batch cultivation.|
|Appears in Collections:||Scopus 2011-2015|
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