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Please use this identifier to cite or link to this item: http://repository.li.mahidol.ac.th/dspace/handle/123456789/35480
Title: Modulating glyoxalase i metal selectivity by deletional mutagenesis: Underlying structural factors contributing to nickel activation profiles
Authors: Uthaiwan Suttisansanee
Yanhong Ran
Kadia Y. Mullings
Nicole Sukdeo
John F. Honek
University of Waterloo
Mahidol University
Jinan University
GreenLight Biosciences
University of Northern British Columbia
Keywords: Biochemistry, Genetics and Molecular Biology;Chemistry;Materials Science
Issue Date: 1-Apr-2015
Citation: Metallomics. Vol.7, No.4 (2015), 605-612
Abstract: © 2015 The Royal Society of Chemistry. Metabolically produced methylglyoxal is a cytotoxic compound that can lead to covalent modification of cellular DNA, RNA and protein. One pathway to detoxify this compound is via the glyoxalase enzyme system. The first enzyme of this detoxification system, glyoxalase I (GlxI), can be divided into two classes according to its metal activation profile, a Zn2+-activated class and a Ni2+-activated class. In order to elucidate some of the key structural features required for selective metal activation by these two classes of GlxI, deletional mutagenesis was utilized to remove, in a step-wise fashion, a key α-helix (residues 73-87) and two small loop regions (residues 99-103 and 111-114) from the Zn2+-activated Pseudomonas aeruginosa GlxI (GloA3) in order to mimic the smaller Ni2+-activated GlxI (GloA2) from the same organism. This approach was observed to clearly shift the metal activation profile of a Zn2+-activated class GlxI into a Ni2+-activated class GlxI enzyme. The α-helix structural component was found to contribute significantly toward GlxI metal specificity, while the two small loop regions were observed to play a more crucial role in the magnitude of the enzymatic activity. The current study should provide additional information on the fundamental relationship of protein structure to metal selectivity in these metalloenzymes.
URI: https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=84927646710&origin=inward
http://repository.li.mahidol.ac.th/dspace/handle/123456789/35480
ISSN: 1756591X
17565901
Appears in Collections:Scopus 2011-2015

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