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Title: Characterization of a subpopulation of developing cortical interneurons from human iPSCs within serum-free embryoid bodies
Authors: Michael W. Nestor
Samson Jacob
Bruce Sun
Deborah Prè
Andrew A. Sproul
Seong Im Hong
Chris Woodard
Matthew Zimmer
Vorapin Chinchalongporn
Ottavio Arancio
Scott A. Noggle
The New York Stem Cell Foundation Research Institute
Columbia University in the City of New York
Hunter College
Mahidol University
Hussman Institute for Autism
Keywords: Biochemistry, Genetics and Molecular Biology
Issue Date: 1-Jan-2015
Citation: American Journal of Physiology - Cell Physiology. Vol.308, No.3 (2015), C209-C219
Abstract: © 2015 the American Physiological Society. Production and isolation of forebrain interneuron progenitors are essential for understanding cortical development and developing cell-based therapies for developmental and neurodegenerative disorders. We demonstrate production of a population of putative calretinin-positive bipolar interneurons that express markers consistent with caudal ganglionic eminence identities. Using serum-free embryoid bodies (SFEBs) generated from human inducible pluripotent stem cells (iPSCs), we demonstrate that these interneuron progenitors exhibit morphological, immunocytochemical, and electrophysiological hallmarks of developing cortical interneurons. Finally, we develop a fluorescence-activated cell-sorting strategy to isolate interneuron progenitors from SFEBs to allow development of a purified population of these cells. Identification of this critical neuronal cell type within iPSC-derived SFEBs is an important and novel step in describing cortical development in this iPSC preparation.
ISSN: 15221563
Appears in Collections:Scopus 2011-2015

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