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|Title:||Characterization of a subpopulation of developing cortical interneurons from human iPSCs within serum-free embryoid bodies|
|Authors:||Michael W. Nestor|
Andrew A. Sproul
Seong Im Hong
Scott A. Noggle
The New York Stem Cell Foundation Research Institute
Columbia University in the City of New York
Hussman Institute for Autism
|Keywords:||Biochemistry, Genetics and Molecular Biology|
|Citation:||American Journal of Physiology - Cell Physiology. Vol.308, No.3 (2015), C209-C219|
|Abstract:||© 2015 the American Physiological Society. Production and isolation of forebrain interneuron progenitors are essential for understanding cortical development and developing cell-based therapies for developmental and neurodegenerative disorders. We demonstrate production of a population of putative calretinin-positive bipolar interneurons that express markers consistent with caudal ganglionic eminence identities. Using serum-free embryoid bodies (SFEBs) generated from human inducible pluripotent stem cells (iPSCs), we demonstrate that these interneuron progenitors exhibit morphological, immunocytochemical, and electrophysiological hallmarks of developing cortical interneurons. Finally, we develop a fluorescence-activated cell-sorting strategy to isolate interneuron progenitors from SFEBs to allow development of a purified population of these cells. Identification of this critical neuronal cell type within iPSC-derived SFEBs is an important and novel step in describing cortical development in this iPSC preparation.|
|Appears in Collections:||Scopus 2011-2015|
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