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Title: International laboratory comparison of influenza microneutralization assays for A(H1N1)pdm09, A(H3N2), and A(H5N1) influenza viruses by CONSISE
Authors: Karen L. Laurie
Othmar G. Engelhardt
John Wood
Alan Heath
Jacqueline M. Katz
Malik Peiris
Katja Hoschler
Olav Hungnes
Wenqing Zhang
Maria D. Van Kerkhove
Jaccqueline Katz
Xiuhua Lu
Min Levine
Vic Veguilla
Feng Liu
Yaohui Bai
Pilaipan Puthavathana
Hatairat Lerdsamran
Phisanu Pooruk
Knnika Nateerom
Maria Rita Castrucci
Isabella Donatelli
Marzia Facchini
Noriko Kishida
Masato Tashiro
Takato Odagiri
Shailesh D. Pawar
Sadhana S. Kode
Anthony Hawksworth
Ryan Ortiguerra
Gary Brice
Nicholas Martin
Tad Kochel
Jose Sanchez
Michael Cooper
James Cummings
Ralf Wagner
Constanze Goepfert
Nina Alex
Joanna Hammann
Britta Neumann
Mahendra Perera
Emanuele Montomoli
Guilia Lapini
Sara Sbragi
Tian Bai
Zaijiang Yu
Jianfang Zhou
Louise Carolan
Karen Laurie
Victorian Infectious Diseases Reference Laboratory
National Institute for Biological Standards and Control
Centers for Disease Control and Prevention
The University of Hong Kong
Public Health England
Norwegian Institute of Public Health
Organisation Mondiale de la Sante
Institut Pasteur, Paris
Mahidol University
Istituto Superiore Di Sanita
National Institute of Infectious Diseases
National Institute of Virology India
U.S. Naval Health Research Center
Naval Medical Research Center
Universita degli Studi di Siena
Chinese National Influenza Center
Keywords: Biochemistry, Genetics and Molecular Biology;Immunology and Microbiology
Issue Date: 1-Jan-2015
Citation: Clinical and Vaccine Immunology. Vol.22, No.8 (2015), 957-964
Abstract: Copyright © 2015, American Society for Microbiology. All Rights Reserved. The microneutralization assay is commonly used to detect antibodies to influenza virus, and multiple protocols are used worldwide. These protocols differ in the incubation time of the assay as well as in the order of specific steps, and even within protocols there are often further adjustments in individual laboratories. The impact these protocol variations have on influenza serology data is unclear. Thus, a laboratory comparison of the 2-day enzyme-linked immunosorbent assay (ELISA) and 3-day hemagglutination (HA) microneutralization (MN) protocols, using A(H1N1)pdm09, A(H3N2), and A(H5N1) viruses, was performed by the CONSISE Laboratory Working Group. Individual laboratories performed both assay protocols, on multiple occasions, using different serum panels. Thirteen laboratories from around the world participated. Within each laboratory, serum sample titers for the different assay protocols were compared between assays to determine the sensitivity of each assay and were compared between replicates to assess the reproducibility of each protocol for each laboratory. There was good correlation of the results obtained using the two assay protocols in most laboratories, indicating that these assays may be interchangeable for detecting antibodies to the influenza A viruses included in this study. Importantly, participating laboratories have aligned their methodologies to the CONSISE consensus 2-day ELISA and 3-day HAMNassay protocols to enable better correlation of these assays in the future.
ISSN: 1556679X
Appears in Collections:Scopus 2011-2015

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