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|Title:||Fast and Simultaneous Analysis of Combined Anti-Diabetic Drugs by Capillary Zone Electrophoresis|
Yvan Vander Heyden
Vrije Universiteit Brussel
|Citation:||Journal of Chromatographic Science. Vol.53, No.6 (2015), 993-999|
|Abstract:||© 2014 The Author 2014. Published by Oxford University Press. All rights reserved. For Permissions, please email: email@example.com. A fast capillary zone electrophoretic method with photodiode array detection (CZE-PAD) was established and validated for assays of commonly prescribed anti-diabetic drugs [metformin (MET), glibenclamide (GBM) and gliclazide (GCZ)] in 13 samples including raw material, single and combined tablets. CZE optimization revealed baseline separation of the analytes (R<inf>s</inf> > 5.39) in 8 min, in 50 mM borate buffer (pH 9.0), using a capillary with an effective length of 56.0 cm and an inner diameter of 50 μm, a voltage of 20 kV, a temperature of 25°C and a detection wavelength at 210 nm. The method provides excellent linearity, precision (%RSDs < 1.90%), recovery (99.8-101.0%) and low detection and quantitation limits (<4 and 12 μg/mL, respectively). The procedure was fast (seven samples per hour) and cost effective, since no organic solvent, sample pre-treatments or clean-up procedures were required. Importantly, the method was accurate, sensitive and reliable for routine quality control of MET, GBM and GCZ in pharmaceutical products both in single and combined formulations.|
|Appears in Collections:||Scopus 2011-2015|
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