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Title: Dual-layered and double-targeted nanogold based lateral flow immunoassay for influenza virus
Authors: Natpapas Wiriyachaiporn
Weerakanya Maneeprakorn
Chayachon Apiwat
Tararaj Dharakul
Thailand National Science and Technology Development Agency
Mahidol University
Keywords: Chemistry
Issue Date: 1-Jan-2015
Citation: Microchimica Acta. Vol.182, No.1-2 (2015), 85-93
Abstract: © Springer-Verlag Wien 2014. We report on a highly sensitive lateral flow immunoassay (LFIA) for influenza A which serves as a model antigen. Gold nanoparticles conjugated to monoclonal antibodies specific to the two most abundant influenza A proteins, nucleoprotein and matrix protein, were used as detector probes. Using this approach, the nucleoprotein and matrix protein in the virion were detected simultaneously. The signal was further amplified via a signal amplification strategy that is making use of two-layered nanogold in combination with a double-targeted detection format. Under optimized conditions, the system is capable of detecting influenza A antigens in infected cells at levels as low as 47 TCID50·mL−1(50 % tissue culture infectious dose) within 15 min. Compared to the conventional LFIA based on single-targeted detection, the detection capability of this system is better by a factor of 8 without requiring additional steps or instruments. In addition to its simplicity and rapidity, this LFIA also can detect the target analyte in even complex biological matrix. This proofof- principle of a dual-layered and double-targeted nanogoldbased LFIA is deemed to be useful for developing single-step, rapid, and sensitive tests for screening and diagnosis.
ISSN: 14365073
Appears in Collections:Scopus 2011-2015

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