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Please use this identifier to cite or link to this item: http://repository.li.mahidol.ac.th/dspace/handle/123456789/35990
Title: Effect of temperature on fimbrial gene expression and adherence of enteroaggregative Escherichia coli
Authors: Woranich Hinthong
Nitaya Indrawattana
Pannamthip Pitaksajjakul
Chonlatip Pipattanaboon
Thida Kongngoen
Prapin Tharnpoophasiam
Suwalee Worakhunpiset
Mahidol University
Keywords: Environmental Science;Medicine
Issue Date: 23-Jul-2015
Citation: International Journal of Environmental Research and Public Health. Vol.12, No.8 (2015), 8631-8643
Abstract: © 2015 by the authors; licensee MDPI, Basel, Switzerland. The influence of temperature on bacterial virulence has been studied worldwide from the viewpoint of climate change and global warming. The bacterium enteroaggregative Escherichia coli (EAEC) is the causative agent of watery diarrhea and shows an increasing incidence worldwide. Its pathogenicity is associated with the virulence factors aggregative adherence fimbria type I and II (AAFI and AAFII), encoded by aggA and aafA in EAEC strains 17-2 and 042, respectively. This study focused on the effect of temperature increases from 29 °C to 40 °C on fimbrial gene expression using real-time PCR, and on its virulence using an aggregative adherence assay and biofilm formation assay. Incubation at 32 °C caused an up-regulation in both EAEC strains 17-2 and strain 042 virulence gene expression. EAEC strain 042 cultured at temperature above 32 °C showed down-regulation of aafA expression except at 38 °C. Interestingly, EAEC cultured at a high temperature showed a reduced adherence to cells and an uneven biofilm formation. These results provide evidence that increases in temperature potentially affect the virulence of pathogenic EAEC, although the response varies in each strain.
URI: https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=84937835174&origin=inward
http://repository.li.mahidol.ac.th/dspace/handle/123456789/35990
ISSN: 16604601
16617827
Appears in Collections:Scopus 2011-2015

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