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Please use this identifier to cite or link to this item: http://repository.li.mahidol.ac.th/dspace/handle/123456789/36020
Title: Development of phenotypic and transcriptional biomarkers to evaluate relative activity of potentially estrogenic chemicals in ovariectomized mice
Authors: Sylvia C. Hewitt
Wipawee Winuthayanon
Brianna Pockette
Robnet T. Kerns
Julie F. Foley
Norris Flagler
Elizabeth Ney
Apichart Suksamrarn
Pawinee Piyachaturawat
Pierre R. Bushel
Kenneth S. Korach
The Research Triangle Park
National Institute of Environmental Health Sciences
Kelly Government Solutions Supporting NTP
Ramkhamhaeng University
Mahidol University
Keywords: Environmental Science
Issue Date: 1-Jan-2015
Citation: Environmental Health Perspectives. Vol.123, No.4 (2015), 344-352
Abstract: © 2015, Public Health Services, US Dept of Health and Human Services. All rights reserved Background: Concerns regarding potential endocrine-disrupting chemicals (EDCs) have led to a need for methods to evaluate candidate estrogenic chemicals. Our previous evaluations of two such EDCs revealed a response similar to that of estradiol (E2) at 2 hr, but a less robust response at 24 hr, similar to the short-acting estrogen estriol (E3). Objectives: Microarray analysis using tools to recognize patterns of response have been utilized in the cancer field to develop biomarker panels of transcripts for diagnosis and selection of treatments most likely to be effective. Biological effects elicited by long- versus short-acting estrogens greatly affect the risks associated with exposures; therefore, we sought to develop tools to predict the ability of chemicals to maintain estrogenic responses. Methods: We used biological end points in uterine tissue and a signature pattern–recognizing tool that identified coexpressed transcripts to develop and test a panel of transcripts in order to classify potentially estrogenic compounds using an in vivo system. The end points used are relevant to uterine tissue, but the resulting classification of the compounds is important for other sensitive tissues and species. results: We evaluated biological and transcriptional end points with proven short- and long-acting estrogens and verified the use of our approach using a phytoestrogen. With our model, we were able to classify the diarylheptanoid D3 as a short-acting estrogen. conclusions: We have developed a panel of transcripts as biomarkers which, together with biological end points, might be used to screen and evaluate potentially estrogenic chemicals and infer mode of activity.
URI: https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=84961289196&origin=inward
http://repository.li.mahidol.ac.th/dspace/handle/123456789/36020
ISSN: 15529924
00916765
Appears in Collections:Scopus 2011-2015

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