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Title: Genetic characterisation of a whiB7 mutant of a Mycobacterium tuberculosis clinical strain
Authors: Saradee Warit
Saranya Phunpruch
Chaitas Jityam
Sarinya Jaitrong
Pamaree Billamas
Angkana Chaiprasert
Prasit Palittapongarnpim
Therdsak Prammananan
Thailand National Center for Genetic Engineering and Biotechnology
King Mongkut's Institute of Technology Ladkrabang
Mahidol University
Keywords: Immunology and Microbiology;Medicine
Issue Date: 1-Dec-2015
Citation: Journal of Global Antimicrobial Resistance. Vol.3, No.4 (2015), 262-266
Abstract: © 2015 International Society for Chemotherapy of Infection and Cancer. Mycobacterium tuberculosis is naturally resistant to clarithromycin (CLR). The genes Rv3197A (whiB7) and Rv1988 (ermMT) have been shown to be involved in the resistant phenotype. In this study, a CLR-susceptible M. tuberculosis clinical strain was identified, designated as DS3214, and the nucleotide sequences and expression profiles of whiB7 and ermMT were investigated. The results revealed that strain DS3214 contained a one nucleotide deletion in whiB7, leading to a truncated peptide. Expression of whiB7 was low, whereas comparable expression of ermMT was determined compared with the reference strain M. tuberculosis H37Rv. Overexpression of the mutant whiB7 in M. tuberculosis H37Ra did not increase the minimum inhibitory concentration (MIC) to CLR or kanamycin, indicating the defect of the mutant WhiB7. The CLR-susceptible M. tuberculosis clinical strain, whose whiB7 is naturally mutated, was first described in this study and whiB7 has been shown to play a role in the CLR-susceptible phenotype.
ISSN: 22137173
Appears in Collections:Scopus 2011-2015

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