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Please use this identifier to cite or link to this item: http://repository.li.mahidol.ac.th/dspace/handle/123456789/36068
Title: The diagnostic accuracy of three rapid diagnostic tests for typhoid fever at Chittagong Medical College Hospital, Chittagong, Bangladesh
Authors: Rapeephan R. Maude
Hanna K. de Jong
Lalith Wijedoru
Masako Fukushima
Aniruddha Ghose
Rasheda Samad
Mohammed Amir Hossain
Mohammed Rezaul Karim
Mohammed Abul Faiz
Christopher M. Parry
Abdullah Abu Sayeed
Mahtab Uddin Hasan
Wirichada Pan-Ngum
Thomas W. van der Vaart
Asok Kumar Dutta
Nasir Uddin Mahmud
Murad Hero
Nafiz Iqbal
Zabeen Chaudhury
Tran Vu Thieu Nga
Pham Thanh Duy
Voong Vinh Phat
Richard J. Maude
Stephen Baker
W. Joost Wiersinga
Tom van der Poll
Nicholas P. Day
Arjen M. Dondorp
Mahidol University
Division of Infectious Diseases and Center for Infection and Immunity Amsterdam (CINIMA)
Academic Medical Centre, University of Amsterdam
Liverpool School of Tropical Medicine
Chittagong Medical College Hospital
Centre for Specialized Care and Research
London School of Hygiene & Tropical Medicine
Nagasaki University
Keywords: Immunology and Microbiology;Medicine
Issue Date: 1-Oct-2015
Citation: Tropical Medicine and International Health. Vol.20, No.10 (2015), 1376-1384
Abstract: © 2015 John Wiley & Sons Ltd. Objective: To determine the diagnostic accuracy of three rapid diagnostic tests (RDTs) for typhoid fever in febrile hospitalised patients in Bangladesh. Methods: Febrile adults and children admitted to Chittagong Medical College Hospital, Bangladesh, were investigated with Bact/Alert® blood cultures and real-time PCR to detect Salmonella enterica Typhi and Paratyphi A and assays for Rickettsia, leptospirosis and dengue fever. Acute serum samples were examined with the LifeAssay (LA) Test-it™ Typhoid IgM lateral flow assay detecting IgM antibodies against S. Typhi O antigen, CTKBiotech Onsite Typhoid IgG/IgM Combo Rapid-test cassette lateral flow assay detecting IgG and IgM antibodies against S. Typhi O and H antigens and SD Bioline line assay for IgG and IgM antibodies against S. Typhi proteins. Results: In 300 malaria smear-negative febrile patients [median (IQR) age of 13.5 (5-31) years], 34 (11.3%) had confirmed typhoid fever: 19 positive by blood culture for S. Typhi (three blood PCR positive) and 15 blood culture negative but PCR positive for S. Typhi in blood. The respective sensitivity and specificity of the three RDTs in patients using a composite reference standard of blood culture and/or PCR-confirmed typhoid fever were 59% and 61% for LifeAssay, 59% and 74% for the CTK IgM and/or IgG, and 24% and 96% for the SD Bioline RDT IgM and/or IgG. The LifeAssay RDT had a sensitivity of 63% and a specificity of 91% when modified with a positive cut-off of ≥2+ and analysed using a Bayesian latent class model. Conclusions: These typhoid RDTs demonstrated moderate diagnostic accuracies, and better tests are needed.
URI: https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=84941040596&origin=inward
http://repository.li.mahidol.ac.th/dspace/handle/123456789/36068
ISSN: 13653156
13602276
Appears in Collections:Scopus 2011-2015

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