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Please use this identifier to cite or link to this item: http://repository.li.mahidol.ac.th/dspace/handle/123456789/36079
Title: Evaluation of polysaccharide-based latex agglutination assays for the rapid detection of antibodies to burkholderia pseudomallei
Authors: Vichaya Suttisunhakul
Narisara Chantratita
Chanthiwa Wikraiphat
Vanaporn Wuthiekanun
Zakiya Douglas
Nicholas P.J. Day
Direk Limmathurotsakul
Paul J. Brett
Mary N. Burtnick
Mahidol University
Keywords: Immunology and Microbiology;Medicine
Issue Date: 1-Sep-2015
Citation: American Journal of Tropical Medicine and Hygiene. Vol.93, No.3 (2015), 542-546
Abstract: Copyright © 2015 by The American Society of Tropical Medicine and Hygiene. Melioidosis is a severe disease caused by the Gram-negative bacterium Burkholderia pseudomallei. Diagnosis of melioidosis currently relies on the isolation of B. pseudomallei from clinical samples, which can take several days. An indirect hemagglutination assay (IHA) is widely used for serodiagnosis, but it has a short shelf life, is poorly standardized, and requires a viable bacteria culture performed in a biosafety level 3 (BSL-3) laboratory. To improve the diagnostic methods, we have developed two rapid latex agglutination tests based on purified B. pseudomallei O-polysaccharide (OPS) and capsular polysaccharide (CPS) antigens. The immunodiagnostic potential of these tests was evaluated using serum from culture-confirmed melioidosis patients (N = 143) and healthy donors from either endemic (N = 199) or nonendemic areas (N = 90). The sensitivity of the OPS-based latex agglutination assay (OPS-latex; 84.4%) was significantly higher than both the CPS-latex (69.5%) (P < 0.001) and IHA (69.5%) (P = 0.001). When evaluated with Thai donor serum, the OPS-latex had comparable specificity (56.9%) to the CPS-latex (63.8%) (P = 0.053), but was significantly lower than the IHA (67.6%) (P = 0.002). In contrast, all tests with U.S. donor serum were highly specific (≥ 97.8%). These results suggest that polysaccharide-based latex agglutination assays may be useful for serodiagnosis of melioidosis in non-endemic areas.
URI: https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=84941628934&origin=inward
http://repository.li.mahidol.ac.th/dspace/handle/123456789/36079
ISSN: 00029637
Appears in Collections:Scopus 2011-2015

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