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Title: Increased intra- and extracellular granzyme expression in patients with tuberculosis
Authors: M. Isabel Garcia-Laorden
Dana C. Blok
Liesbeth M. Kager
Arie J. Hoogendijk
Gerard J. Van Mierlo
Ivar O. Lede
Wahid Rahman
Rumana Afroz
Aniruddha Ghose
Caroline E. Visser
Abu Shahed Md Zahed
Md Anwar Husain
Khan Mashrequl Alam
Pravat Chandra Barua
Mahtabuddin Hassan
Ahmed Hossain
Md Abu Tayab
Nick Day
Arjen M. Dondorp
Alex F. De Vos
Tom Van Der Poll
Academic Medical Centre, University of Amsterdam
Sanquin Research
Chittagong Medical College Hospital
National TB Control Programme
Chest Disease Clinic Chittagong (CDCC)
Chittagong General Hospital
Mahidol University
Keywords: Immunology and Microbiology;Medicine
Issue Date: 1-Sep-2015
Citation: Tuberculosis. Vol.95, No.5 (2015), 575-580
Abstract: © 2015 Elsevier Ltd. Summary Tuberculosis (TB) is an important cause of morbidity and mortality worldwide. Granzymes (gzms) are proteases mainly found in cytotoxic lymphocytes, but also extracellularly. While the role of gzms in target cell death has been widely characterized, considerable evidence points towards broader roles related to infectious and inflammatory responses. To investigate the expression of the gzms in TB, intracellular gzms A, B and K were measured by flow cytometry in lymphocyte populations from peripheral blood mononuclear cells from 18 TB patients and 12 healthy donors from Bangladesh, and extracellular levels of gzmA and B were measured in serum from 58 TB patients and 31 healthy controls. TB patients showed increased expression of gzmA in CD8+ T, CD4+ T and CD56+ T, but not NK, cells, and of gzmB in CD8+ T cells, when compared to controls. GzmK expression was not altered in TB patients in any lymphocyte subset. The extracellular levels of gzmA and, to a lesser extent, of gzmB, were increased in TB patients, but did not correlate with intracellular gzm expression in lymphocyte subsets. Our results reveal enhanced intra- and extracellular expression of gzmA and B in patients with pulmonary TB, suggesting that gzms are part of the host response to tuberculosis.
ISSN: 1873281X
Appears in Collections:Scopus 2011-2015

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