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Title: Development of a blocking latex agglutination test for the detection of antibodies to chicken anemia virus
Authors: Dai Quang Trinh
Haruko Ogawa
Vuong Nghia Bui
Tham Thi Hong Nguyen
Dulyatad Gronsang
Tugsbaatar Baatartsogt
Mugimba Kahoza Kizito
Mohammed AboElkhair
Shigeo Yamaguchi
Viet Khong Nguyen
Kunitoshi Imai
Obihiro University of Agriculture and Veterinary Medicine
National Institute of Veterinary Research Hanoi
Mahidol University
Makerere University
The University of Sadat City
Japan Livestock Technology Association
Keywords: Immunology and Microbiology
Issue Date: 4-May-2015
Citation: Journal of Virological Methods. Vol.221, (2015), 74-80
Abstract: © 2015 Elsevier B.V. A blocking latex agglutination test (b-LAT) developed in this study was evaluated for the detection of antibodies against chicken anemia virus (CAV) in chickens. Polystyrene latex beads were coupled with a neutralizing monoclonal antibody (mAb) to CAV (mAb-beads). When mAb-beads were mixed with antigens prepared from the lysate of MDCC-MSB1 cells infected with CAV, agglutination occurred. A short pre-incubation of CAV antigens with CAV-specific antiserum inhibited the agglutination of mAb-beads. The test results were obtained within 5. min. The specificity of b-LAT was evaluated using sera from specific pathogen-free chickens and sera containing antibodies to avian influenza virus, Newcastle disease virus, infectious bursal disease virus, and Marek's disease virus; nonspecific agglutination and cross-reactivity with antibodies to unrelated viruses were not observed. The examination of 94 serum samples collected from commercial breeder chickens of various ages (17-63 weeks) revealed good agreement (93.6%, Kappa value. =. 0.82) between b-LAT and a virus neutralization test, known to be most sensitive and specific in the detection of antibodies to CAV. These results indicate that b-LAT, a simple and rapid test, is a useful and reliable tool in CAV serology.
ISSN: 18790984
Appears in Collections:Scopus 2011-2015

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