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dc.contributor.authorPornanan Kueakhaien_US
dc.contributor.authorNarin Changklungmoaen_US
dc.contributor.authorKulathida Chaithirayanonen_US
dc.contributor.authorManussabhorn Phatsaraen_US
dc.contributor.authorNarin Preyavichyapugdeeen_US
dc.contributor.authorSuda Riengrojpitaken_US
dc.contributor.authorKant Sangpairojen_US
dc.contributor.authorPhiraphol Chusongsangen_US
dc.contributor.authorPrasert Sobhonen_US
dc.contributor.otherMahidol Universityen_US
dc.contributor.otherBurapha Universityen_US
dc.contributor.otherChiang Mai Universityen_US
dc.contributor.otherSilpakorn Universityen_US
dc.identifier.citationExperimental Parasitology. Vol.151-152, (2015), 8-13en_US
dc.description.abstract© 2015 Elsevier Inc. Saposin-like protein 2 (SAP-2) plays an important role in the digestive process of Fasciola gigantica (Fg). It is one of the major proteins synthesized by the caecal epithelial cells and released into fluke's excretion-secretion. Therefore, FgSAP-2 is a plausible target for detecting fasciolosis. A polyclonal antibody (PoAb) against recombinant FgSAP-2 was produced by immunizing rabbits with the recombinant protein (rFgSAP-2), and used in sandwich ELISA assay to detect the circulating FgSAP-2 in sera of mice experimentally infected with F. gigantica metacercariae. The assay could detect rFgSAP-2 and the native FgSAP-2 in the excretory-secretory (ES) and whole body (WB) fractions of adult F. gigantica at the concentrations as low as 38 pg/ml, 24 ng/ml, and 102 ng/ml, respectively. As well, the sera from mice experimentally infected with F. gigantica were tested positive by this sandwich ELISA, which exhibited sensitivity, specificity, false positive rate, false negative rate and accuracy at 99.99, 98.67, 1.33, 0.01 and 99.32%, respectively. Therefore, this assay could be used for diagnosis of fasciolosis by F. gigantica.en_US
dc.rightsMahidol Universityen_US
dc.subjectImmunology and Microbiologyen_US
dc.titleSaposin-like protein 2 has an immunodiagnostic potential for detecting Fasciolosis giganticaen_US
Appears in Collections:Scopus 2011-2015

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