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Title: IscR plays a role in oxidative stress resistance and pathogenicity of a plant pathogen, Xanthomonas campestris
Authors: Mayuree Fuangthong
Thichakorn Jittawuttipoka
Ratiphorn Wisitkamol
Adisak Romsang
Jintana Duang-nkern
Paiboon Vattanaviboon
Skorn Mongkolsuk
Chulabhorn Research Institute
Chulabhorn Graduate Institute
Mahidol University
Center for Environmental Health
Keywords: Immunology and Microbiology
Issue Date: 1-Jan-2015
Citation: Microbiological Research. Vol.170, (2015), 139-146
Abstract: © 2014 Elsevier GmbH. Iron-sulfur ([Fe-S]) cluster is an essential cofactor of proteins involved in various physiological processes including cellular defense against oxidative stress. In Xanthomonas campestris pv. campestris (Xcc), IscR plays a negative role in regulation of the transcription of [Fe-S] assembly genes, iscR-sufBCDS. The expression level of sufBCDS was up-regulated in an Xcc iscR mutant. In addition, the iscR promoter activity in an Xcc iscR mutant was also higher than the wild-type strain, indicating an autoregulatory circuit. Purified IscR was shown to bind at the iscR promoter region and three putative IscR binding sites were identified. The expression of iscR-suf operon was highly induced by oxidant treatments and iron limited conditions. The iscR mutant showed increased sensitivity toward hydrogen peroxide phenotype but, surprisingly, had hyper-resistant phenotype toward plumbagin compared to the wild-type strain. Most importantly, the iscR mutant was impaired in its ability to cause lesion on leaves of a compatible host plant, Chinese radish (Raphanus sativus). These results demonstrate that a transcription regulator gene, iscR, negatively regulates genes involved in [Fe-S] biosynthesis and plays a role in oxidative stress response and pathogenesis of Xcc.
ISSN: 09445013
Appears in Collections:Scopus 2011-2015

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