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Please use this identifier to cite or link to this item: http://repository.li.mahidol.ac.th/dspace/handle/123456789/36284
Title: T-Cell Responses Are Associated with Survival in Acute Melioidosis Patients
Authors: Kemajittra Jenjaroen
Suchintana Chumseng
Manutsanun Sumonwiriya
Pitchayanant Ariyaprasert
Narisara Chantratita
Piyanate Sunyakumthorn
Maliwan Hongsuwan
Vanaporn Wuthiekanun
Helen A. Fletcher
Prapit Teparrukkul
Direk Limmathurotsakul
Nicholas P.J. Day
Susanna J. Dunachie
Mahidol University
London School of Hygiene & Tropical Medicine
Sappasithiprasong Hospitali
University of Oxford
Keywords: Medicine
Issue Date: 23-Oct-2015
Citation: PLoS Neglected Tropical Diseases. Vol.9, No.10 (2015)
Abstract: © 2015 Jenjaroen et al. Background: Melioidosis is an increasingly recognised cause of sepsis and death across South East Asia and Northern Australia, caused by the bacterium Burkholderia pseudomallei. Risk factors include diabetes, alcoholism and renal disease, and a vaccine targeting at-risk populations is urgently required. A better understanding of the protective immune response in naturally infected patients is essential for vaccine design. Methods: We conducted a longitudinal clinical and immunological study of 200 patients with melioidosis on admission, 12 weeks (n = 113) and 52 weeks (n = 65) later. Responses to whole killed B. pseudomallei were measured in peripheral blood mononuclear cells (PBMC) by interferon-gamma (IFN-γ) ELIspot assay and flow cytometry and compared to those of control subjects in the region with diabetes (n = 45) and without diabetes (n = 43). Results: We demonstrated strong CD4+ and CD8+ responses to B. pseudomallei during acute disease, 12 weeks and 52 weeks later. 28-day mortality was 26% for melioidosis patients, and B. pseudomallei-specific cellular responses in fatal cases (mean 98 IFN-γ cells per million PBMC) were significantly lower than those in the survivors (mean 142 IFN-γ cells per million PBMC) in a multivariable logistic regression model (P = 0.01). A J-shaped curve association between circulating neutrophil count and mortality was seen with an optimal count of 4000 to 8000 neutrophils/μl. Melioidosis patients with known diabetes had poor diabetic control (median glycated haemoglobin HbA1c 10.2%, interquartile range 9.2–13.1) and showed a stunted B. pseudomallei-specific cellular response during acute illness compared to those without diabetes. Conclusions: The results demonstrate the role of both CD4+ and CD8+ T-cells in protection against melioidosis, and an interaction between diabetes and cellular responses. This supports development of vaccine strategies that induce strong T-cell responses for the control of intracellular pathogens such as B. pseudomallei.
URI: https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=84959194792&origin=inward
http://repository.li.mahidol.ac.th/dspace/handle/123456789/36284
ISSN: 19352735
19352727
Appears in Collections:Scopus 2011-2015

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