Please use this identifier to cite or link to this item: http://repository.li.mahidol.ac.th/dspace/handle/123456789/36314
Title: Comparison between flow cytometry, microscopy, and lactate dehydrogenase-based enzyme-linked immunosorbent assay for plasmodium falciparum drug susceptibility testing under field conditions
Authors: Charles J. Woodrow
Chirapat Wangsing
Kanlaya Sriprawat
Peter R. Christensen
Francois Nosten
Laurent Rénia
Bruce Russell
Benoît Malleret
Nuffield Department of Clinical Medicine
Mahidol University
Agency for Science, Technology and Research, Singapore
Yong Loo Lin School of Medicine
Keywords: Medicine
Issue Date: 1-Oct-2015
Citation: Journal of Clinical Microbiology. Vol.53, No.10 (2015), 3296-3303
Abstract: Copyright © 2015, American Society for Microbiology. All Rights Reserved. Flow cytometry is an objective method for conducting in vitro antimalarial sensitivity assays with increasing potential for application in field sites. We examined in vitro susceptibility to seven anti-malarial drugs for 40 fresh P. falciparum field isolates via a flow cytometry method (FCM), a colorimetric LDH-based ELISA (DELI), and standard microscopic slide analysis of growth. For FCM, 184/280 (66%) assays met analytical acceptance criteria, compared to 166/280 (59%) for DELI. There was good agreement between FCM and microscopy, while DELI tended to produce higher half-maximal inhibition constants (IC50s) than FCM, with an overall bias of 2.2-fold (Bland-Altman comparison). Values for artesunate and dihydroartemisinin were most affected. Paradoxical increases in signal at very high concentrations of mefloquine and related compounds were more marked with the DELI assay, suggesting that off-target effects on LDH production may be responsible. Loss of FCM signal due to reinvasion or slow growth was observed in a small number of samples. These results extend previous work on use of flow cytometry to determine antimalarial susceptibility in terms of the number of samples, range of drugs, and comparison with other methods.
URI: https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=84941949667&origin=inward
http://repository.li.mahidol.ac.th/dspace/handle/123456789/36314
ISSN: 1098660X
00951137
Appears in Collections:Scopus 2011-2015

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