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dc.contributor.authorCharles J. Woodrowen_US
dc.contributor.authorChirapat Wangsingen_US
dc.contributor.authorKanlaya Sriprawaten_US
dc.contributor.authorPeter R. Christensenen_US
dc.contributor.authorFrancois Nostenen_US
dc.contributor.authorLaurent Réniaen_US
dc.contributor.authorBruce Russellen_US
dc.contributor.authorBenoît Mallereten_US
dc.contributor.otherNuffield Department of Clinical Medicineen_US
dc.contributor.otherMahidol Universityen_US
dc.contributor.otherAgency for Science, Technology and Research, Singaporeen_US
dc.contributor.otherYong Loo Lin School of Medicineen_US
dc.identifier.citationJournal of Clinical Microbiology. Vol.53, No.10 (2015), 3296-3303en_US
dc.description.abstractCopyright © 2015, American Society for Microbiology. All Rights Reserved. Flow cytometry is an objective method for conducting in vitro antimalarial sensitivity assays with increasing potential for application in field sites. We examined in vitro susceptibility to seven anti-malarial drugs for 40 fresh P. falciparum field isolates via a flow cytometry method (FCM), a colorimetric LDH-based ELISA (DELI), and standard microscopic slide analysis of growth. For FCM, 184/280 (66%) assays met analytical acceptance criteria, compared to 166/280 (59%) for DELI. There was good agreement between FCM and microscopy, while DELI tended to produce higher half-maximal inhibition constants (IC50s) than FCM, with an overall bias of 2.2-fold (Bland-Altman comparison). Values for artesunate and dihydroartemisinin were most affected. Paradoxical increases in signal at very high concentrations of mefloquine and related compounds were more marked with the DELI assay, suggesting that off-target effects on LDH production may be responsible. Loss of FCM signal due to reinvasion or slow growth was observed in a small number of samples. These results extend previous work on use of flow cytometry to determine antimalarial susceptibility in terms of the number of samples, range of drugs, and comparison with other methods.en_US
dc.rightsMahidol Universityen_US
dc.titleComparison between flow cytometry, microscopy, and lactate dehydrogenase-based enzyme-linked immunosorbent assay for plasmodium falciparum drug susceptibility testing under field conditionsen_US
Appears in Collections:Scopus 2011-2015

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