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Title: Anti-amebic activity of diosgenin on Naegleria Fowleri trophozoites
Authors: Jundee Rabablert
Supathra Tiewcharoen
Prasert Auewarakul
Thassanant Atithep
Natchagorn Lumlerdkij
Renu Vejaratpimol
Virach Junnu
Silpakorn University
Mahidol University
Keywords: Medicine
Issue Date: 1-Jan-2015
Citation: Southeast Asian Journal of Tropical Medicine and Public Health. Vol.46, No.5 (2015), 827-834
Abstract: © 2015, SEAMEO TROPMED Network. All rights reserved. The aim of this study was to investigate the activity of diosgenin against Naegleria fowleri trophozoites at the cellular and molecular levels. Diosgenin (100 μg/ml; 241.2 μM) had a 100% inhibitory effect on N. fowleri trophozoites (5x105cell/ml). Scanning electron micrograph revealed diosgenin decreased the number of sucker-like apparatuses and food cup formation among N. fowleri trophozoites at 3 and 6 hours post-exposure, respectively. Diosgenin down-regulated the nf cysteine protease gene expression of N. fowleri trophozoites at 6 and 12 hours post-exposure. The toxicity to mammalian cells caused by diosgenin at therapeutic dose was less than amphotericin B, the current drug used to treat N. fowleri infections. Our findings suggest diosgenin has activity against the surface membrane and the nf cysteine protease of N. fowleri trophozoites. However, the other mechanisms of action of diosgenin against N. fowleri trophozoites require further exploration.
ISSN: 01251562
Appears in Collections:Scopus 2011-2015

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