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Title: Buffer-optimized high gradient magnetic separation: Target cell capture efficiency is predicted by linear bead-capture theory
Authors: Shahid Waseem
Rachanee Udomsangpetch
Sebastian C. Bhakdi
Mahidol University
Quaid-i-Azam University
X-Zell Biotec Co.
Keywords: Engineering;Materials Science
Issue Date: 1-Jan-2016
Citation: Journal of Magnetics. Vol.21, No.1 (2016), 125-132
Abstract: © The Korean Magnetics Society. All rights reserved. High gradient magnetic separation (HGMS) is the most commonly used magnetic cell separation technique in biomedical science. However, parameters determining target cell capture efficiencies in HGMS are still not well understood. This limitation leads to loss of information and resources. The present study develops a beadcapture theory to predict capture efficiencies in HGMS. The theory is tested with CD3- and CD14-positive cells in combination with paramagnetic beads of different sizes and a generic immunomagnetic separation system. Data depict a linear relationship between normalized capture efficiency and the bead concentration. In addition, it is shown that key biological functions of target cells are not affected for all bead sizes and concentrations used. In summary, linear bead-capture theory predicts capture efficiency (Et) in a highly significant manner.
ISSN: 12261750
Appears in Collections:Scopus 2016-2017

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