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dc.contributor.authorPaweena Chaoprasiden_US
dc.contributor.authorThanittra Dokpikulen_US
dc.contributor.authorJaruwan Johnroden_US
dc.contributor.authorSirin Sirirakphaisarnen_US
dc.contributor.authorSumontha Nookabkaewen_US
dc.contributor.authorRojana Sukchawaliten_US
dc.contributor.authorSkorn Mongkolsuken_US
dc.contributor.otherChulabhorn Research Instituteen_US
dc.contributor.otherChulabhorn Graduate Instituteen_US
dc.contributor.otherThailand Ministry of Educationen_US
dc.contributor.otherMahidol Universityen_US
dc.date.accessioned2018-12-11T01:59:08Z
dc.date.accessioned2019-03-14T08:01:39Z-
dc.date.available2018-12-11T01:59:08Z
dc.date.available2019-03-14T08:01:39Z-
dc.date.issued2016-06-01en_US
dc.identifier.citationApplied and Environmental Microbiology. Vol.82, No.12 (2016), 3503-3514en_US
dc.identifier.issn10985336en_US
dc.identifier.issn00992240en_US
dc.identifier.other2-s2.0-84971401596en_US
dc.identifier.urihttps://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=84971401596&origin=inwarden_US
dc.identifier.urihttp://repository.li.mahidol.ac.th/dspace/handle/123456789/40759-
dc.description.abstract© 2016, American Society for Microbiology. Agrobacterium tumefaciens has a cluster of genes (Atu3178, Atu3179, and Atu3180) encoding an ABC-type transporter, here named troA, troB, and troC, respectively, which is shown here to be a zinc-specific uptake system. Reverse transcription (RT)-PCR analysis confirmed that troA, troB, and troC are cotranscribed, with troC as the first gene of the operon. The yciC (Atu3181) gene is transcribed in the opposite orientation to that of the troCBA operon and belongs to a metal-binding GTPase family. Expression of troCBA and yciC was inducible under zinc-limiting conditions and was controlled by the zinc uptake regulator, Zur. Compared to the wild type, the mutant strain lacking troC was hypersensitive to a metal chelator, EDTA, and the phenotype could be rescued by the addition of zinc, while the strain with a single yciC mutation showed no phenotype. However, yciC was important for survival under zinc limitation when either troC or zinT was inactivated. The periplasmic zinc-binding protein, ZinT, could not function when TroC was inactivated, suggesting that ZinT may interact with TroCBA in zinc uptake. Unlike many other bacteria, the ABC-type transporter ZnuABC was not the major zinc uptake system in A. tumefaciens. However, the important role of A. tumefaciens ZnuABC was revealed when TroCBA was impaired. The strain containing double mutations in the znuA and troC genes exhibited a growth defect in minimal medium. A. tumefaciens requires cooperation of zinc uptake systems and zinc chaperones, including TroCBA, ZnuABC, ZinT, and YciC, for survival under a wide range of zinc-limiting conditions.en_US
dc.rightsMahidol Universityen_US
dc.source.urihttps://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=84971401596&origin=inwarden_US
dc.subjectAgricultural and Biological Sciencesen_US
dc.subjectBiochemistry, Genetics and Molecular Biologyen_US
dc.subjectEnvironmental Scienceen_US
dc.subjectImmunology and Microbiologyen_US
dc.titleAgrobacterium tumefaciens Zur regulates the high-affinity zinc uptake system TroCBA and the putative metal chaperone YciC, along with ZinT and ZnuABC, for survival under zinc-limiting conditionsen_US
dc.typeArticleen_US
dc.rights.holderSCOPUSen_US
dc.identifier.doi10.1128/AEM.00299-16en_US
Appears in Collections:Scopus 2016-2017

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