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Please use this identifier to cite or link to this item: http://repository.li.mahidol.ac.th/dspace/handle/123456789/40782
Title: Cellufine sulfate column chromatography as a simple, rapid, and effective method to purify dengue virus
Authors: Rattiyaporn Kanlaya
Visith Thongboonkerd
Mahidol University
Keywords: Immunology and Microbiology
Issue Date: 1-Aug-2016
Citation: Journal of Virological Methods. Vol.234, (2016), 174-177
Abstract: © 2016 Elsevier B.V. Conventional method to purify/concentrate dengue virus (DENV) is time-consuming with low virus recovery yield. Herein, we applied cellufine sulfate column chromatography to purify/concentrate DENV based on the mimicry between heparan sulfate and DENV envelope protein. Comparative analysis demonstrated that this new method offered higher purity (as determined by less contamination of bovine serum albumin) and recovery yield (as determined by greater infectivity). Moreover, overall duration used for cellufine sulfate column chromatography to purify/concentrate DENV was approximately 1/20 of that of conventional method. Therefore, cellufine sulfate column chromatography serves as a simple, rapid, and effective alternative method for DENV purification/concentration.
URI: https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=84965052635&origin=inward
http://repository.li.mahidol.ac.th/dspace/handle/123456789/40782
ISSN: 18790984
01660934
Appears in Collections:Scopus 2016-2017

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