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dc.contributor.authorSuchada Sumruaypholen_US
dc.contributor.authorChamnarn Apiwathnasornen_US
dc.contributor.authorJiraporn Ruangsittichaien_US
dc.contributor.authorPatchara Sriwichaien_US
dc.contributor.authorSiriluck Attrapadungen_US
dc.contributor.authorYudthana Samungen_US
dc.contributor.authorJean Pierre Dujardinen_US
dc.contributor.otherMahidol Universityen_US
dc.contributor.otherCIRAD Centre de Recherche de Montpellieren_US
dc.identifier.citationActa Tropica. Vol.159, (2016), 1-10en_US
dc.description.abstract© 2016 Elsevier B.V. Aedes aegypti (Diptera: Culicidae) (L.), Ae. albopictus (Skuse), and Ae. scutellaris (Walker) are important mosquito vectors of dengue and chikungunya viruses. They are morphologically similar and sympatric in some parts of their distribution; therefore, there is a risk of incorrect morphological identification. Any confusion could have a negative impact on epidemiological studies or control strategies. Therefore, we explored two modern tools to supplement current morphological identification: DNA barcoding and geometric morphometric analyses. Field larvae were reared to adults and carefully classified based on morphological traits. The genetic analysis was based on the 658 bp each of 30COI sequences. Some Culex spp., Mansonia bonneae, were included as outgroups, and inclusion of a few other Aedes spp. facilitated phylogenetic inference of the relationship between Ae. albopictus and Ae. scutellaris. The two species were separated by an average interspecific divergence of 0.123 (0.119-0.127). Morphometric examination included landmark- (392 specimens) and outline-based (317 specimens) techniques. The shape of the wing showed different discriminating power based on sex and digitizing technique. This is the first time that Ae. scutellaris and Ae. albopictus have been compared using these two techniques. We confirm that these morphologically close species are valid, and that geometric morphometrics can considerably increase the reliability of morphological identification.en_US
dc.rightsMahidol Universityen_US
dc.subjectImmunology and Microbiologyen_US
dc.titleDNA barcoding and wing morphometrics to distinguish three Aedes vectors in Thailanden_US
Appears in Collections:Scopus 2016-2017

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