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Please use this identifier to cite or link to this item: http://repository.li.mahidol.ac.th/dspace/handle/123456789/40823
Title: Neuraminidase activity and resistance of 2009 pandemic H1N1 influenza virus to antiviral activity in bronchoalveolar fluid
Authors: Kanyarat Ruangrung
Ornpreya Suptawiwat
Kittipong Maneechotesuwan
Chompunuch Boonarkart
Warunya Chakritbudsabong
Jirawatna Assawabhumi
Parvapan Bhattarakosol
Mongkol Uiprasertkul
Pilaipan Puthavathana
Witthawat Wiriyarat
Anan Jongkaewwattana
Prasert Auewarakul
Mahidol University
Chulalongkorn University
Thailand National Center for Genetic Engineering and Biotechnology
Keywords: Immunology and Microbiology
Issue Date: 1-May-2016
Citation: Journal of Virology. Vol.90, No.9 (2016), 4637-4646
Abstract: © 2016, American Society for Microbiology. All Rights Reserved. Human bronchoalveolar fluid is known to have anti-influenza activity. It is believed to be a frontline innate defense against the virus. Several antiviral factors, including surfactant protein D, are believed to contribute to the activity. The 2009 pandemic H1N1 influenza virus was previously shown to be less sensitive to surfactant protein D. Nevertheless, whether different influenza virus strains have different sensitivities to the overall anti-influenza activity of human bronchoalveolar fluid was not known. We compared the sensitivities of 2009 pandemic H1N1, seasonal H1N1, and seasonal H3N2 influenza virus strains to inhibition by human bronchoalveolar lavage (BAL) fluid. The pandemic and seasonal H1N1 strains showed lower sensitivity to human BAL fluid than the H3N2 strains. The BAL fluid anti-influenza activity could be enhanced by oseltamivir, indicating that the viral neuraminidase (NA) activity could provide resistance to the antiviral defense. In accordance with this finding, the BAL fluid antiinfluenza activity was found to be sensitive to sialidase. The oseltamivir resistance mutation H275Y rendered the pandemic H1N1 virus but not the seasonal H1N1 virus more sensitive to BAL fluid. Since only the seasonal H1N1 but not the pandemic H1N1 had compensatory mutations that allowed oseltamivir-resistant strains to maintain NA enzymatic activity and transmission fitness, the resistance to BAL fluid of the drug-resistant seasonal H1N1 virus might play a role in viral fitness.
URI: https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=84964990174&origin=inward
http://repository.li.mahidol.ac.th/dspace/handle/123456789/40823
ISSN: 10985514
0022538X
Appears in Collections:Scopus 2016-2017

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