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Title: The evaluation of loop-mediated isothermal amplification-quartz crystal microbalance (LAMP-QCM) biosensor as a real-time measurement of HPV16 DNA
Authors: Patcharee Jearanaikoon
Preeda Prakrankamanant
Chanvit Leelayuwat
Surasak Wanram
Temduang Limpaiboon
Chamras Promptmas
Khon Kaen University
Ubon Rajathanee University
Mahidol University
Keywords: Immunology and Microbiology
Issue Date: 1-Mar-2016
Citation: Journal of Virological Methods. Vol.229, (2016), 8-11
Abstract: © 2015 Elsevier B.V. We have previously developed quartz crystal microbalance biosensor integrated with loop-mediated isothermal amplification (LAMP-QCM) for human papillomavirus (HPV) type58 DNA detection. Infection with HPV, particularly HPV16, remains a serious health problem due to its major risk factor contributing to cervical cancer. In the present study, LAMP-QCM biosensor was evaluated in terms of a quantitative assay for copy number of HPV16 DNA in cervical samples compared to quantitative PCR using TaqMan assay (TaqMan-qPCR). The detection limit of LAMP-QCM was found to be 10 fold more sensitive than TaqMan-qPCR with 100% specificity and 7.6% imprecision. Different plot of HPV16 DNA copy number using Bland-Altman analysis revealed 94% correlation between LAMP-QCM and qPCR. We therefore concluded that the developed LAMP-QCM biosensor provides a possible rapid and sensitive assay for HPV16 DNA quantification in a routine laboratory.
ISSN: 18790984
Appears in Collections:Scopus 2016-2017

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